Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate), Powder

Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) is a broad-spectrum aminoglycoside antibiotic used for selecting genetically modified cells. It is produced by the bacterium Micromonospora rhodorangea and functions by binding to ribosomal subunits, inhibiting protein synthesis in both prokaryotic and eukaryotic cells.

Resistance to G418 Sulfate is conferred by the E. coli APH (3’) – I and APH (3’) – II resistance genes, making it a widely used selection agent for stable cell line generation in molecular biology and genetic engineering research.

This powdered formulation allows researchers to prepare custom stock solutions ranging from 10 to 50 mg/mL in sterile water, offering flexibility in concentration adjustments based on experimental needs.

Key Features & Benefits

✅ High-Efficiency Selection – Eliminates non-resistant cells for stable transfection studies

✅ Flexible Concentration Range – Customizable stock solution (10–50 mg/mL in water)

✅ Broad-Spectrum Activity – Effective for mammalian, insect, and bacterial cell culture

✅ Sterile, High-Purity Powder – Ensures optimal performance and reproducibility

✅ cGMP-Compliant & ISO 13485 Certified – Manufactured under strict quality standards

✅ Dual-Site Manufacturing – Ensures uninterrupted supply and consistency

Applications

🔬 Stable Cell Line Generation – Selection of genetically modified cells carrying a resistance gene

🦠 Prokaryotic & Eukaryotic Selection – Effective for both bacterial and mammalian cell culture

🧬 Gene Expression Studies – Used in experiments requiring stable gene expression

🛠️ Biotechnology & Pharmaceutical Research – Essential for genetic engineering and drug discovery

Quality & Manufacturing Standards

Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) is manufactured under rigorous quality control procedures to ensure consistent and reliable performance.

cGMP Manufacturing & ISO 13485 Certification

• Manufactured at two facilities – Grand Island, NY (USA) & Scotland, UK
• Certified to ISO 13485 – Meets stringent quality requirements for laboratory reagents
• FDA-registered medical device manufacturing sites
• Batch-to-batch consistency ensures reproducibility across experiments

Product Specifications

Usage Guidelines

• Recommended Working Concentrations:
  • Bacteria: 100–200 µg/mL
  • Mammalian Cells: 200–500 µg/mL
• Stock Solution Preparation:
  • Dissolve powder in sterile water to prepare a 10–50 mg/mL stock solution
  • Store stock solutions at –20°C for long-term stability
  • Avoid autoclaving the solution to preserve antibiotic activity

Ordering Information

Shelf Life: 24 months from the date of manufacture.

Regulatory & Safety Information

⚠️ For Research Use Only. Not intended for diagnostic, therapeutic, or human/animal use.

• Always follow laboratory safety protocols.
• Refer to the Safety Data Sheet (SDS) for proper handling and disposal guidelines.

Why Choose Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate), Powder?

🔹 High-Purity & Sterile Powder – Enables flexible concentration adjustments

🔹 Reliable & Consistent Selection – Trusted for stable cell line generation

🔹 Dual-Site cGMP Manufacturing – Reduces supply chain risks

🔹 Validated for Research Applications – Ideal for genetic studies, molecular biology, and biotechnology

For bulk orders, technical support, or additional information, contact LabMart Limited today.