Gibco™ DPBS, calcium, magnesium

The Gibco™ Dulbecco's Phosphate-Buffered Saline (DPBS) is a high-quality balanced salt solution designed to support various cell culture applications. Enhanced with calcium and magnesium and free from phenol red, this DPBS formulation ensures reliable performance in processes such as cell washing, transportation, and reagent preparation.

Key Features:

1. Optimized Formulation:
   • Contains calcium and magnesium, which are essential for cell-to-cell adhesion and stability during sample handling.
   • Free from phenol red to avoid interference in sensitive experiments or imaging applications.
2. Reliable Performance:
   • Sterile-filtered to ensure contamination-free use in laboratory workflows.
   • Maintains physiological pH (7.0–7.2) and osmolality (270–310 mOsm/kg) for compatibility with cell culture systems.
3. Versatile Applications:
   • Ideal for washing cells before enzymatic dissociation.
   • Useful for transporting cells, tissues, or organoids.
   • Supports reagent preparation and dilution of cell suspensions for counting.
4. Manufacturing and Compliance:
   • Manufactured in a cGMP-compliant facility adhering to ISO 13485 standards, ensuring consistent quality.
   • Suitable for research use and further manufacturing; not for diagnostic or therapeutic applications.
5. Convenient Packaging:
   • Available in multiple volumes to meet diverse laboratory needs, ranging from 100 mL to 1000 mL and multipack options.

Specifications:

Applications:

1. Cell Culture Preparation:
   • Washes cells to remove residual serum and other debris before enzymatic dissociation.
   • Maintains cell health and viability during experimental handling.
2. Cell and Tissue Transport:
   • Provides a stable environment for transporting cells or tissue samples between laboratory locations or during experiments.
3. Dilution and Reagent Preparation:
   • Dilutes cell suspensions for counting and downstream applications.
   • Prepares reagents for molecular and cellular workflows.
4. Imaging Studies:
   • Suitable for imaging workflows where phenol red interference must be avoided.

Packaging Options:

Advantages:

1. High Quality and Sterility:
   • Ensures contamination-free usage for reproducible results.
2. Customizable Packaging:
   • Various volume options to accommodate small-scale experiments or large-scale workflows.
3. Research-Grade Versatility:
   • Adaptable to numerous cell culture and molecular biology applications.
4. Reliable Storage:
   • Long shelf life of 36 months under recommended conditions for flexibility in storage and use.

Usage Instructions:

1. Store the product at 2°C to 8°C.
2. Use sterile techniques when handling to prevent contamination.
3. For cell washing, ensure the solution is at the desired temperature before use.

The Gibco™ DPBS with Calcium and Magnesium is an essential tool for cell culture applications, ensuring stability and reproducibility in various workflows. Its high-quality manufacturing and versatile application make it a trusted solution for researchers in academic, clinical, and industrial laboratories.