Gibco™ DMEM, high glucose, 1X

Widely Trusted Basal Medium for Robust Mammalian Cell Growth

Gibco™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose is a widely used, nutrient-rich basal medium formulated to support the growth of a broad range of mammalian cell types. This DMEM formulation contains 4.5 g/L glucose, L-glutamine, and phenol red, and is free of sodium pyruvate and HEPES, making it ideal for cell culture workflows that require defined supplementation and controlled conditions.

Originally developed as an enhancement to Eagle’s Minimal Essential Medium (MEM), DMEM offers four times the concentration of amino acids and vitamins, supporting enhanced cell growth, viability, and metabolic activity.

Key Features & Benefits

• High Glucose Formulation (4.5 g/L)
  Supports high-energy-demanding cell types and long-term cultures.
• L-Glutamine Included
  Provides a readily available source of nitrogen to support protein and nucleotide biosynthesis.
• Phenol Red Indicator
  Enables real-time monitoring of pH shifts in the medium.
• Animal-Origin Free & Sterile-Filtered
  Manufactured under cGMP-compliant, ISO 13485-certified conditions to ensure consistency and safety.
• Versatile Application
  Suitable for a wide variety of primary cells and immortalized cell lines, including HeLa, HEK293, Cos-7, PC-12, as well as neurons, fibroblasts, glial cells, HUVECs, and smooth muscle cells.
• Standard Supplementation Ready
  Requires serum supplementation, commonly 10% Fetal Bovine Serum (FBS), as it contains no proteins or growth factors.

Recommended Applications

• Mammalian cell culture expansion
• Primary cell isolation and maintenance
• Transfection and expression studies
• Recombinant protein and viral production
• Drug screening and toxicity testing

Specifications Table

Usage Notes

1. Supplementation: Add 10% FBS or other appropriate serum before use.
2. CO₂ Environment: Maintain a 5–10% CO₂ atmosphere in the incubator to preserve physiological pH.
3. Storage: Store at 2–8°C and protect from light. Do not freeze.
4. Sterility: Use aseptic technique to maintain sterility during dispensing and culture.

Gibco™ DMEM, High Glucose is a foundational cell culture medium optimized to support robust mammalian cell proliferation and maintenance. Trusted globally for its consistency, quality, and compatibility with a wide range of cell types, this formulation provides researchers with a flexible and dependable solution for routine and advanced cell culture applications.