Gibco™ Collagenase, Type I, powder from Clostridium histolyticum

Gibco™ Collagenase Type I is a protease enzyme that uniquely cleaves the bond between a neutral amino acid (X) and glycine in the Pro-X-Gly-Pro sequence, found abundantly in collagen. This enzyme is essential for degrading triple-helical native collagen fibrils found in connective tissues, such as skin, tendon, blood vessels, and bone. It is widely used for tissue and cell dissociation in various research applications.

Applications:

• Tissue Disaggregation: Suitable for culturing:
  • Human tumors.
  • Mouse kidney.
  • Human adult and fetal brain.
  • Epithelial tissues.
• Organ Perfusions: Useful for liver, adrenal, and fat tissue digestion.
• Gentle Dissociation: Operates at physiological temperature and pH without requiring mechanical agitation or special equipment.

Key Features:

1. High Activity:
   • Guaranteed activity of greater than 125 units/mg.
   • Effective in dissociating tissues without damaging cells.
2. Versatile Enzyme Profile:
   • Average levels of collagenase, caseinase, clostripain, and tryptic activities.
   • Optimized for fat, adrenal, and liver tissue digestion.
3. Convenient Format:
   • Lyophilized powder for easy handling and storage.
   • Non-sterile, research-grade enzyme.

Specifications:

• Form: Lyophilized powder.
• Sterility: Non-sterile.
• Shelf Life: 24 months from the date of manufacture.
• Activity: ≥125 units/mg.
• Recommended Storage: 2°C to 8°C; protect from light.

Product Details:

• Catalog Numbers and Sizes:
  • 17100017: 1 g.
  • 17018029: 500 mg.
• Shipping Conditions: Room temperature.
• For Research Use Only: Not for diagnostic or therapeutic purposes.

Advantages:

• Efficient Tissue Digestion: Collagenase's unique ability to degrade native collagen fibrils makes it ideal for a range of tissues.
• Broad Applicability: Works well with many cell types and tissues, including human, mouse, and other mammalian sources.
• Stable and Easy to Use: Long shelf life and stable activity simplify experimental workflows.

Ordering Information:

• Catalog Numbers:
  • 17100017 (1 g).
  • 17018029 (500 mg).