Thermo Scientific™ HhaI (10 U/μL)

The Thermo Scientific™ HhaI Restriction Enzyme is a high-quality endonuclease that recognizes and cleaves DNA at the sequence GCG^C. Optimized for reliable performance, HhaI operates best at 37°C using the Tango buffer and is a critical tool for molecular biology applications such as cloning, genotyping, and restriction mapping.

Key Features:

1. Recognition and Cleavage Site:
   • Recognizes the palindromic sequence GCG^C and generates a 4-base sticky end.
   • Cleavage produces cohesive ends ideal for cloning and other downstream applications.
2. Optimized Buffer System:
   • Works efficiently in Tango buffer, a universal buffer compatible with many enzymes for convenient double-digestion protocols.
3. High Quality:
   • Manufactured under stringent quality controls to ensure consistent activity and specificity.
4. Methylation Sensitivity:
   • Sensitive to CpG methylation, allowing selective digestion of unmethylated DNA.
   • Not sensitive to Dam or Dcm methylation.
5. Convenient Packaging:
   • Supplied at a concentration of 10 U/µL, with a total of 2,000 units per vial.

Applications:

1. Molecular Cloning:
   • Facilitates the creation of recombinant DNA by generating compatible cohesive ends for ligation.
2. Restriction Site Mapping:
   • Identifies and maps restriction sites in DNA for sequencing or structural studies.
3. Genotyping:
   • Detects polymorphisms in DNA sequences for genetic analysis.
4. Southern Blotting:
   • Prepares DNA samples for hybridization and probe detection.
5. RFLP (Restriction Fragment Length Polymorphism):
   • Analyzes genetic variations by comparing fragment patterns after digestion.
6. SNP (Single Nucleotide Polymorphism) Detection:
   • Identifies single base changes in DNA sequences through digestion patterns.

Specifications:

Reaction Setup:

1. Components:
   • DNA: Up to 1 µg.
   • HhaI Restriction Enzyme: 1 µL (10 U/µL) per reaction.
   • 10X Tango Buffer: 2 µL.
   • Water: Adjust to 20 µL total reaction volume.
2. Protocol:
   • Incubate at 37°C for 1–16 hours, depending on DNA complexity and reaction conditions.
   • Analyze digestion products via gel electrophoresis.

Advantages:

1. Superior Quality:
   • Rigorous quality control ensures high specificity, reliable cleavage, and minimal star activity.
2. Buffer Convenience:
   • Works with the Five Buffer System for simplified reaction optimization and compatibility with other enzymes.
3. Versatile Applications:
   • Supports a wide range of molecular biology workflows, including cloning and SNP detection.
4. Efficient Workflow:
   • Pre-mixed BSA in reaction buffers enhances stability and reduces handling time.

Storage and Handling:

• Store at -20°C for long-term stability.
• Avoid repeated freeze-thaw cycles to maintain enzyme activity.

Product Details:

The Thermo Scientific™ HhaI Restriction Enzyme is a versatile and high-quality tool for molecular biology applications. Its robust performance, methylation sensitivity, and optimized buffer system make it indispensable for precise and efficient DNA manipulation.