Thermo Scientific™ S1 Nuclease (100 U/μL)

The Thermo Scientific™ S1 Nuclease is a single-strand–specific endonuclease that selectively degrades single-stranded nucleic acids (DNA and RNA), releasing 5′-phosphoryl mono- and oligonucleotides. With approximately five times greater activity on DNA than RNA, it is a powerful tool for nucleic acid research and genome editing workflows.

In addition to single-stranded substrates, S1 Nuclease cleaves double-stranded DNA (dsDNA) at single-stranded regions, such as nicks, gaps, mismatches, or loops, and exhibits 3′-phosphomonoesterase activity. The enzyme is a glycoprotein containing 18% carbohydrate, contributing to its structural stability and functionality.

Key Features

• High Specificity – Efficiently degrades single-stranded DNA and RNA.
• Dual Activity – Cleaves dsDNA at single-stranded regions caused by nicks, gaps, loops, or mismatches.
• Versatile Research Use – Removes ssDNA overhangs, maps RNA transcripts, and resolves DNA hairpins.
• Enzyme Synergy – Can be combined with Exonuclease III to create unidirectional deletions in DNA fragments.
• Reliable Activity – Exhibits 3′-phosphomonoesterase activity for additional nucleic acid processing.

Applications

• Removal of single-stranded overhangs from DNA fragments
• S1 nuclease transcript mapping
• Cleavage of hairpin loops in DNA
• Generation of unidirectional deletions (in combination with Exonuclease III)
• Detection and analysis of mismatched or looped DNA regions

Technical Specifications

Ordering Information

The Thermo Scientific™ S1 Nuclease is a highly active, single-strand–specific endonuclease ideal for applications such as overhang removal, transcript mapping, hairpin cleavage, and unidirectional deletion mutagenesis. Its ability to also cleave mismatched or looped regions in dsDNA makes it a versatile enzyme for molecular biology, cloning, and genetic research.