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Invitrogen™ Reduced Glutathione (GSH) Colorimetric Assay Kit, 96 tests
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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Reduced Glutathione (GSH) Colorimetric Assay Kit is a highly sensitive and reliable tool for the quantitative measurement of reduced glutathione (GSH) in biological samples. GSH is a critical intracellular antioxidant that supports cellular redox balance, protects against oxidative stress, and plays an important role in cardiovascular, neurological, and cancer-related research.
This kit is designed for use in serum, plasma, cell culture supernatants, tissue homogenates, and whole cells, offering consistent, reproducible, and rapid results.
Now includes access to our FREE online Reduced Glutathione (GSH) Colorimetric Assay Kit Calculator, which automatically calculates GSH concentrations from your OD450 readings, saving you analysis time and ensuring accuracy.
Principle of the Assay
Reduced GSH reacts with dinitrobenzoic acid (DTNB) to form a yellow complex measurable at 450 nm using a microplate reader. The free calculator allows you to input absorbance values and instantly generate concentration results based on your standard curve.
Key Features
- Rapid and User-Friendly – Results in as little as 25 minutes.
- High Sensitivity – Detects as low as 2 μmol/L.
- Free GSH Calculator – Automates data analysis for quick and accurate results.
- Broad Sample Compatibility – Multiple biological sample types supported.
- Strict QC Standards – Reliable reproducibility with low intra/interassay variability.
Applications
- Oxidative Stress Research
- Cardiovascular Disease Studies (atherosclerosis, coronary heart disease)
- Cancer Research – Tumor redox analysis
- Neurodegenerative Studies (Alzheimer’s, Parkinson’s)
- Pharmaceutical Antioxidant Screening
Technical Specifications
| Parameter | Specification |
|---|---|
| Assay Range | 2–100 μmol/L |
| Assay Sensitivity | 2 μmol/L |
| Detection Method | Colorimetric (450 nm) |
| Total Assay Time | 25 minutes |
| Incubation Time | 5 minutes |
| Sample Volume | 100 μL |
| Sample Types | Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates, Whole Cells |
| Intraassay CV | 1.9% |
| Interassay CV | 3.2% |
| For Use With | Microplate Reader |
| Shipping Condition | Dry Ice, 2–8°C |
| Storage | 2–8°C |
The Invitrogen™ Reduced Glutathione (GSH) Colorimetric Assay Kit, combined with our free online GSH Calculator, delivers fast, precise, and user-friendly glutathione quantification for cutting-edge research in oxidative stress and related diseases.