Invitrogen™ Glutathione Peroxidase (GSH-Px) Activity Kit, 96 Tests

Reliable colorimetric assay for quantifying GSH-Px enzyme activity in diverse biological samples

The Invitrogen™ Glutathione Peroxidase (GSH-Px) Activity Kit provides a highly sensitive and reproducible method for quantifying GSH-Px activity in cell culture supernatants, plasma, serum, tissue homogenates, and whole cells. GSH-Px is a critical antioxidant enzyme that protects cells from oxidative damage by catalyzing the reduction of hydrogen peroxide and organic hydroperoxides, using reduced glutathione (GSH) as a cofactor.

In this assay, GSH-Px activity is determined by measuring the consumption of GSH. Reduced glutathione reacts with dinitrobenzoic acid (DTNB) to produce 5-thio-dinitrobenzoic acid anion, a stable yellow product. The absorbance of this product is measured at 412 nm to calculate GSH levels, which correlate with enzyme activity.

Key Features

• High Sensitivity – Detects activity as low as 17.17 U/L
• Wide Assay Range – 17.17–518.32 U/L for flexible sample measurement
• Fast and Easy Protocol – Minimal hands-on time, total assay time of 40 minutes
• Broad Sample Compatibility – Works with cell culture supernatants, plasma, serum, tissues, and whole cells
• Reproducible Results – Low intra-assay CV (2.4%) and inter-assay CV (6.2%)

Applications

• Oxidative stress studies and antioxidant profiling
• Metabolic pathway research involving reactive oxygen species
• Toxicology testing for oxidative injury assessment
• Disease model research where oxidative damage is implicated
• Screening of compounds that influence GSH-Px activity

Assay Principle

1. GSH-Px in the sample catalyzes the reduction of hydrogen peroxide or organic hydroperoxides using GSH as a cofactor.
2. The residual GSH reacts with DTNB, producing a yellow-colored 5-thio-dinitrobenzoic acid anion.
3. The absorbance at 412 nm is proportional to the amount of residual GSH, which inversely reflects GSH-Px activity.
4. The Glutathione Peroxidase (GSH-Px) Activity Kit Calculator is typically used to convert your absorbance readings at 412 nm into GSH-Px enzyme activity based on the standard curve you generate during the assay.

Specifications

The Invitrogen™ Glutathione Peroxidase (GSH-Px) Activity Kit offers a fast, sensitive, and reliable method for measuring GSH-Px activity in a variety of biological samples. With its easy-to-use protocol and broad sample compatibility, it is ideal for oxidative stress research, antioxidant screening, and disease biomarker studies.