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Invitrogen™ EnzChek™ Cellulase Substrate, blue fluorescent, 339/452, 1mg
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Specifications:
| Application | Fluorescence-Based Assay | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Molecular Biology Reagent, Enzymes and Substrates | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ EnzChek™ Cellulase Substrate is a simple, rapid, and highly sensitive assay substrate for the quantitative measurement of cellulase activity. Designed for both routine and high-throughput applications, this substrate enables accurate results in 30 minutes or less with minimal steps.
Unlike complex, multistep cellulase assays, the EnzChek™ substrate requires only enzyme addition, incubation, and fluorescence or absorbance reading. This makes it ideal for laboratories looking to increase throughput without compromising sensitivity.
Principle of the Assay
The assay uses a blue-fluorescent substrate with excitation/emission maxima at ~339/452 nm. In the presence of cellulase, the substrate is hydrolyzed, releasing highly fluorescent fragments proportional to enzyme activity.
While optimized for fluorescence detection (providing maximum sensitivity, as low as 40 μU/mL cellulase), the substrate can also be used in a colorimetric assay with slightly reduced sensitivity.
Key Features
- Fast Results – Complete cellulase quantitation in 30 minutes or less.
- High Sensitivity – Detects as little as 40 μU/mL cellulase.
- Flexible Detection – Works with both fluorescence and colorimetric readouts.
- High-Throughput Ready – Streamlined protocol ideal for screening applications.
- Simple Workflow – Single incubation step with minimal sample preparation.
Applications
- Enzyme Kinetics – Measure cellulase activity over time.
- Industrial Biotechnology – Screen cellulase for biofuel production.
- Agricultural Research – Study plant cell wall degradation.
- Food Science – Assess cellulase in food processing.
- Microbial Enzyme Studies – Evaluate cellulase-producing microorganisms.
Technical Specifications
| Parameter | Specification |
|---|---|
| Detection Method | Fluorescence (~339/452 nm) or Colorimetric |
| Form | Solid |
| Substrate Type | Cellulase substrate |
| Substrate Properties | Chemical-based |
| Assay Time | ≤ 30 minutes |
| Sensitivity | 40 μU/mL cellulase |
| For Use With | Fluorometer, Microplate Reader |
| Shipping Condition | Room Temperature |
| Storage | –5°C to –30°C, protect from light |
| Quantity | 1 mg |
| Product Line | EnzChek™ |
The Invitrogen™ EnzChek™ Cellulase Substrate is an excellent choice for researchers who require fast, sensitive, and reproducible cellulase activity measurements in a high-throughput or standard laboratory environment. Its dual detection capability ensures flexibility, while its simple workflow makes it suitable for routine use in industrial, agricultural, and academic applications.