R&D Stop Solution 2N Sulfuric Acid, 15 x 6mL

The R&D Systems Stop Solution (2N Sulfuric Acid) is specifically formulated for terminating the enzymatic reaction of horseradish peroxidase (HRP) on tetramethylbenzidine (TMB) substrate in ELISA assays. This solution ensures consistent and reliable results by stabilizing the color change at the endpoint, allowing accurate optical density (OD) measurements.

Product Highlights

1. Pre-Measured Convenience:
   • Supplied in 15 vials, each containing 6 mL of 2N sulfuric acid for precise use in ELISA procedures.
2. Specific Use in ELISA:
   • Designed to stop HRP-TMB reactions, stabilizing the colorimetric output for consistent OD readings at 450 nm.
3. Storage Stability:
   • Stable for up to 30 days after opening when stored at 2–8 °C.

Key Specifications

• Part Number: 895032
• Quantity: 15 vials, 6 mL per vial
• Concentration: 2N Sulfuric Acid
• Application: ELISA
• Storage Temperature: 2–8 °C
• Shelf Life: Use before the expiration date indicated on the label.

Procedure for Use

1. Stopping the Reaction:
   • Add 50–100 µL of the Stop Solution to each well after the desired incubation time with the TMB substrate.
2. Mixing:
   • If the color appears non-uniform or green, gently tap the microplate to ensure thorough mixing.
3. Measurement:
   • Measure the optical density (OD) at 450 nm using a plate reader.

Applications

• ELISA Assays:
  • Stop HRP-catalyzed reactions on TMB substrate to stabilize the colorimetric signal.
• Research and Diagnostics:
  • Used in laboratories conducting ELISA for protein quantification, antibody detection, or diagnostic purposes.

Storage & Stability

• Unopened Product: Store at 2–8 °C.
• Opened Product: Stable for up to 30 days when stored at 2–8 °C and handled appropriately.
• Shipping Conditions: Shipped at ambient temperature.

Safety Instructions

• Contains 2N Sulfuric Acid, a corrosive substance.
• Protective Measures:
  • Wear gloves, protective clothing, and eye protection.
  • Wash hands thoroughly after handling.
• Disposal: Dispose of according to local regulations for hazardous materials.
• Material Safety Data Sheet (MSDS): Available on the R&D Systems website for detailed handling and hazard information.

Limitations & Technical Hints

• Ensure uniform mixing to achieve consistent color across the wells.
• Avoid over-incubation with TMB before adding the Stop Solution, as this may lead to signal variability.
• Do not use past the expiration date for optimal performance.

Why Choose This Product?

1. Ready-to-Use Convenience: Pre-measured vials simplify ELISA workflows and ensure consistent reagent quality.
2. High Compatibility: Designed to work seamlessly with HRP-TMB substrates in ELISA applications.
3. Reliable Performance: Stabilizes the reaction for accurate OD readings and reproducible results.
4. Trusted Quality: Manufactured by R&D Systems, a leader in reliable research reagents.

This Stop Solution is a critical component for any laboratory performing ELISA assays, ensuring precision and reproducibility in each experiment.