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Specifications:
| Application | PCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ dNTP Mix (10 mM each) contains premixed aqueous solutions of the four deoxynucleotide triphosphates (dNTPs): dATP, dCTP, dGTP, and dTTP. Each nucleotide is provided at a final concentration of 10 mM, offering a convenient, pre-mixed solution to reduce pipetting steps and minimize the risk of reaction setup errors. This dNTP mix is designed for use in a variety of molecular biology applications, including PCR, RT-PCR, qPCR, cDNA synthesis, and DNA sequencing.
The dNTPs in this mix are greater than 99% pure, free of nuclease activities, and do not contain human or E. coli DNA. This ensures high-quality and consistent results across experiments, with long-term stability, even after multiple freeze-thaw cycles.
Features
- High Purity: Greater than 99% purity, confirmed by HPLC, ensuring high-quality performance in PCR and other applications.
- Nuclease-Free: Free from nuclease activities, human, and E. coli DNA, eliminating the risk of contamination in experiments.
- Long-Term Stability: The dNTPs remain stable, with up to 95% of the dNTPs remaining in triphosphate form after 7 weeks at room temperature.
- Efficient in PCR: Up to 90% of the dNTPs remain in triphosphate form after 30 cycles of PCR (1 minute at 94°C, 3 minutes at 72°C), ensuring robust amplification.
Applications
- Long Range PCR (up to 40 kb): Ideal for long-range PCR, amplifying large DNA fragments.
- cDNA Synthesis: Used in the synthesis of complementary DNA (cDNA) from RNA templates.
- Standard PCR, RT-PCR, and qPCR: Supports a wide range of PCR-based applications, including reverse transcription and quantitative PCR (qPCR).
- High-Fidelity PCR: Suitable for high-fidelity PCR applications that require accuracy and reduced error rates.
- DNA Labeling and Sequencing: Can be used in DNA labeling and sequencing protocols to ensure accurate incorporation of nucleotides.
- LAMP, RDA, MDA: Also compatible with LAMP, RDA, and MDA techniques.
Specifications
| Property | Details |
|---|---|
| Form | Liquid |
| Label or Dye | Unlabeled |
| Purity or Quality Grade | >99% (confirmed by HPLC) |
| Shipping Condition | Dry Ice |
| For Use With | Standard PCR, High-fidelity PCR, RT-PCR, Real Time PCR (qPCR) |
| Concentration | 10 mM (each dNTP) |
| Unit Size | Available in sizes: 1 mL, 0.2 mL, 5 x 1 mL, 10 x 1 mL |
| Storage Conditions | Store at –20°C |
Contents
- dNTP Mix (10 mM each): Contains dATP, dCTP, dGTP, and dTTP in equal concentrations of 10 mM each.
Storage and Handling
- Storage: Store the dNTP mix at –20°C to maintain its stability and effectiveness.
- Shipping: The product is shipped with dry ice to maintain its integrity during transport.
Important Notes
- For Research Use Only: This product is intended only for research purposes and is not for diagnostic use.
- Freeze-Thaw: The product has long-term stability, but multiple freeze-thaw cycles should be avoided to maintain optimal performance.
This dNTP Mix (10 mM each) simplifies and enhances the accuracy of your PCR-based experiments by providing high-purity nucleotides in a convenient premixed format. It ensures minimal setup errors and high-quality amplification for a wide variety of applications
- Pack Size: 1 mL 5 x 1 mL 10 x 1 mL 0.2 mL