Cyclosporin A, 99.98%

Cyclosporin A is a high-purity (99.98%) cyclic oligopeptide immunosuppressant that binds to cyclophilin and inhibits calcineurin with an IC₅₀ of 7 nM in a cell-free assay. This inhibition prevents T-cell activation by blocking nuclear translocation of NF-AT (nuclear factor of activated T cells), making it essential for organ transplantation, autoimmune disease research, and inflammation studies.

Additionally, Cyclosporin A (NSC 290193) is widely used in cancer research, mitochondrial studies, and neuroprotection models. It is also employed in animal models for chronic rejection of liver transplantation.

Key Features & Benefits

1. High-Purity Immunosuppressant for Research

• 99.98% purity ensures reliable and reproducible experimental results.
• Selective binding to cyclophilin, inhibiting calcineurin and T-cell activation.
• Prevents graft rejection and suppresses immune responses.

2. Proven Mechanism in Immune Suppression & Inflammatory Regulation

• Blocks interleukin-2 (IL-2) transcription, preventing T-cell proliferation.
• Prevents mitochondrial permeability transition pore (MTP) opening (IC₅₀ = 39 nM).
• Inhibits pro-inflammatory cytokines (IFN-γ, TNF-α), reducing immune activation.

3. Versatile Research Applications (In Vitro & In Vivo Studies)

• Studied in transplantation models for immunosuppression research.
• Used in neuroprotection models for ischemia-reperfusion injury.
• Evaluates apoptosis and immune cell regulation in cancer studies.

4. Ready-to-Use for Laboratory Experiments

• Available in various quantities (10 mM solution, 50 mg, 200 mg, 1 g).
• Soluble in DMSO, ethanol, and methanol for flexible experimental use.
• Long-term stability when stored at -20°C in powder form.

Mechanism of Action

In Vitro Studies

• Binds to cyclophilin, forming a Cyclosporin A-cyclophilin complex.
• Inhibits calcineurin, preventing NF-AT activation and IL-2 transcription.
• Suppresses T-cell activation and immune responses.

In Vivo Studies

• Reduces immune response and inflammation in transplantation models.
• Neuroprotective effects in stroke models, preventing neuronal apoptosis.
• Modulates mitochondrial function, preventing oxidative stress-induced cell death.

Chemical & Solubility Information

Clinical & Research Applications

1. Transplantation & Autoimmune Disease Research

• Prevention of organ transplant rejection (kidney, liver, heart, lung).
• Suppression of autoimmune responses in lupus, rheumatoid arthritis, and psoriasis.

2. Cancer Immunotherapy & Tumor Biology

• Study of calcineurin-NFAT signaling in cancer progression.
• Evaluation of Cyclosporin A in drug-resistant cancer cells.

3. Neuroprotection & Ischemia Research

• Prevention of neuronal apoptosis in ischemia-reperfusion models.
• Potential therapeutic applications in neurodegenerative diseases (Parkinson’s, Alzheimer’s, ALS).

4. Inflammation & Mitochondrial Dysfunction Studies

• Inhibition of mitochondrial permeability transition pore (MTP) opening.
• Reduction of inflammatory cytokines in chronic disease models.

Clinical Trial Information

Storage & Handling

• Store powder at -20°C for long-term stability.
• Prepare stock solutions in DMSO, ethanol, or methanol.
• Aliquot stock solutions to avoid repeated freeze-thaw cycles.

Ordering Information

Selleckchem Cyclosporin A, 99.98%, is a high-purity immunosuppressant used in transplantation, autoimmune disease research, neuroprotection, and mitochondrial function studies. Its calcineurin inhibition and mitochondrial regulation make it a valuable tool for immunology, cancer therapy, and inflammation research.