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Thermo Scientific™ Oxoid™ Haemophilus Test Medium Base (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Haemophilus Test Medium Base (Dehydrated) is a highly specialized medium designed for susceptibility testing of Haemophilus influenzae and other Haemophilus species. Based on the formulation developed by Jorgensen et al. and recommended by the CLSI M100Ed 29, this medium provides highly reproducible results while maintaining transparency for easy reading of inhibition zones during antimicrobial testing.
Key Features
- Designed for Haemophilus Susceptibility Testing:
- Optimized for testing Haemophilus influenzae and other nutritionally fastidious Haemophilus species.
- Transparent Medium:
- Allows clear visualization of inhibition zones through the bottom of the Petri dish, enhancing accuracy.
- Low Antagonist Levels:
- Formulated to minimize the antagonistic effects of nutrients on antimicrobial agents, enabling confident testing of trimethoprim/sulphamethoxazole and other antibiotics.
- Reproducible Results:
- Provides consistent and reliable results for routine antimicrobial susceptibility testing.
- CLSI Recommended:
- Complies with the guidelines specified by the Clinical and Laboratory Standards Institute (CLSI) for Haemophilus testing.
Applications
1. Clinical Microbiology
- Susceptibility testing of Haemophilus influenzae and other Haemophilus species from clinical specimens.
- Antibiotic susceptibility testing for respiratory tract infections caused by Haemophilus spp.
2. Diagnostic Testing
- Supports workflows for determining effective antimicrobial treatments against Haemophilus-related infections.
3. Research Microbiology
- Studies on antibiotic resistance and susceptibility patterns in Haemophilus species.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Dehydrated Culture Media |
| Form | Powder |
| Description | Haemophilus Test Medium Base |
| Applications | Clinical, diagnostic, and research microbiology |
| Quantity | 500g |
| Yield | 11.6L of prepared medium |
Preparation Instructions
- Suspend: Suspend 43g of Haemophilus Test Medium Base powder in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Dispense: Pour into sterile Petri dishes and allow to solidify.
Advantages
- Transparency for Accuracy: Enables clear and accurate reading of inhibition zones.
- High Reproducibility: Delivers consistent results for routine susceptibility testing.
- Antagonist-Free: Allows confident testing of antibiotics like trimethoprim/sulphamethoxazole.
- Regulatory Compliance: Meets CLSI recommendations, ensuring reliable performance in diagnostic workflows.
- Cost-Effective: High yield of 11.6L per 500g, suitable for routine use in clinical laboratories.
Diagnostic Features
| Application | Outcome |
|---|---|
| Susceptibility Testing | Clear zones of inhibition for antimicrobial evaluation |
| Haemophilus Growth | Supports reliable growth for accurate testing |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to product label for expiration date.
Packaging
- Product: Haemophilus Test Medium Base (Dehydrated)
- Catalog Number: CM0898B
- Quantity: 500g
The Thermo Scientific™ Oxoid™ Haemophilus Test Medium Base (Dehydrated) is a reliable and effective medium for susceptibility testing of Haemophilus influenzae and other fastidious species. Its transparent formulation, reproducibility, and CLSI compliance make it a valuable tool for clinical laboratories focused on delivering accurate antimicrobial susceptibility results.