Thermo Scientific™ Oxoid™ Violet Red Bile Agar (VRBA, Dehydrated)

The Thermo Scientific™ Oxoid™ Violet Red Bile Agar (Dehydrated) is a selective medium designed for the detection and enumeration of coliform organisms in water, food, and dairy products. This lactose-containing medium is widely used in the microbiological examination of food, dairy equipment, and environmental samples for quality assurance and spoilage potential. Its selective properties inhibit non-target Gram-positive bacteria, ensuring accurate and reliable results.

Key Features

1. Selective for Coliforms:
   • Contains crystal violet and bile salts to suppress the growth of Gram-positive bacteria.
2. Lactose Fermentation Indicator:
   • Coliforms rapidly ferment lactose, forming red-purple colonies with red-purple halos.
   • Late lactose fermenters form pale colonies with greenish halos.
3. Versatile Applications:
   • Suitable for water, milk, dairy products, food samples, and environmental testing.
4. Widely Trusted:
   • Recognized by APHA standards for the microbiological examination of dairy products, food, and water.

Specifications

Applications

1. Water Testing:
   • Detects coliforms in drinking water, wastewater, and environmental water samples.
2. Food Microbiology:
   • Monitors coliform contamination in milk, dairy products, and processed foods.
3. Quality Assurance:
   • Assesses food quality and spoilage potential through the presence of indicator organisms.
4. Environmental Monitoring:
   • Evaluates hygiene and microbial contamination in production facilities and equipment.

Preparation Instructions

1. Suspend 41.5g of Violet Red Bile Agar powder in 1 liter of distilled water.
2. Heat gently to dissolve completely.
3. Bring to boil while stirring to avoid localized overheating.
4. Do not autoclave. Cool to 45-50°C, pour into sterile Petri dishes, and allow to set.

Advantages

• Highly Selective: Crystal violet and bile salts effectively suppress non-target organisms.
• Accurate Results: Rapid lactose fermentation produces easily interpretable red-purple colonies.
• Versatile Use: Applicable for food, water, and environmental microbiology.
• Validated: Trusted by APHA standards for quality microbiological testing.

Packaging

• Quantity: 500g of dehydrated powder.
• Yield: Prepares 13.0L of medium.

Why Choose Violet Red Bile Agar?

• Trusted medium for coliform detection and enumeration in food safety workflows.
• Ideal for microbiological testing in diverse industries, including food, water, and dairy.
• Backed by Thermo Scientific’s commitment to quality and innovation.