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Thermo Scientific™ Oxoid™ Kligler Iron Agar (Dehydrated), 500G
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Kligler Iron Agar (Dehydrated) is a differential medium used for the identification of Enterobacteriaceae based on double sugar fermentation (glucose and lactose) and hydrogen sulfide (H₂S) production. This medium combines the features of Kligler's Lead Acetate medium and Russell's Double Sugar Agar and is recommended for identifying colonies isolated on plating media such as MacConkey Agar, Bismuth Sulphite Agar, or Desoxycholate Citrate Agar.
Key Features
- Differential Medium:
- Differentiates Enterobacteriaceae based on sugar fermentation and H₂S production.
- Versatile Application:
- Ideal for identifying colonies isolated from selective media, including clinical and non-clinical specimens.
- Distinct Reactions:
- Differentiates lactose fermenters, glucose fermenters, and non-fermenters based on color changes in the medium.
- H₂S Detection:
- Incorporates ferric ions for the detection of hydrogen sulfide production, indicated by blackening in the medium.
- Reliable Performance:
- Provides clear and reproducible results for routine microbiological workflows.
Applications
1. Clinical Microbiology
- Identification of Enterobacteriaceae from clinical specimens, including stool, urine, and blood cultures.
2. Food and Environmental Microbiology
- Testing for Salmonella, Shigella, and other Enterobacteriaceae in food, water, and environmental samples.
3. Research Microbiology
- Studies on sugar fermentation patterns and H₂S production in Gram-negative enteric bacteria.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Agar |
| Form | Powder |
| Description | Kligler Iron Agar |
| Applications | Clinical, food, and environmental microbiology |
| Quantity | 500g |
| Yield | 9.1L of prepared medium |
Preparation Instructions
- Suspend: Suspend 55g of Kligler Iron Agar powder in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved.
- Dispense: Dispense the medium into test tubes.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Solidify: Allow the medium to solidify in a slanted position.
Advantages
- Differential Utility: Clearly identifies lactose fermenters, glucose fermenters, and non-fermenters.
- H₂S Production: Effectively detects hydrogen sulfide-producing organisms with visible blackening.
- Wide Applicability: Suitable for use in clinical, food, and environmental microbiology.
- Cost-Effective: High yield of 9.1L per 500g, ideal for routine laboratory testing.
Typical Reactions on Kligler Iron Agar
| Organism | Slant | Butt | Gas | H₂S |
|---|---|---|---|---|
| Shigella sonnei | Red | Yellow | - | - |
| Salmonella typhi | Red | Yellow | - | + |
| Escherichia coli | Yellow | Yellow | + | - |
| Proteus mirabilis | Red | Yellow | - | + |
| Citrobacter freundii | Yellow | Yellow | + | + |
| Enterobacter species | Yellow | Yellow | + | - |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to product label for expiration date.
Packaging
- Product: Kligler Iron Agar (Dehydrated)
- Catalog Number: CM0033B
- Quantity: 500g
- Yield: 9.1L of prepared medium
The Thermo Scientific™ Oxoid™ Kligler Iron Agar (Dehydrated) is a reliable and versatile medium for identifying Enterobacteriaceae based on sugar fermentation and H₂S production. Its ability to differentiate organisms based on distinct color changes and gas production makes it an indispensable tool for clinical, food, and environmental microbiology laboratories.