Thermo Scientific™ Oxoid™ Nutrient Agar (Dehydrated), 500G

The Thermo Scientific™ Oxoid™ Nutrient Agar (Dehydrated) is a versatile, non-selective microbiological medium used for the cultivation and maintenance of a wide range of microorganisms. Its balanced formula provides essential nutrients required for bacterial growth, making it a staple medium for general microbiological purposes, including teaching, research, and clinical applications. This medium is particularly suited for subculturing, maintaining control strains, and preparing organisms for biochemical and serological tests.

Key Features

1. Comprehensive Nutrient Composition:
   • Contains beef extract, peptone, yeast extract, and sodium chloride to supply nitrogen, carbon, vitamins, and trace nutrients.
   • Supports the growth of non-fastidious microorganisms and can be enriched with up to 10% blood or biological fluids for specific needs.
2. Versatile Applications:
   • Ideal for cultivating and maintaining microorganisms from clinical, environmental, and industrial samples.
   • Suitable for creating semi-solid agar slopes or agar butts to maintain control organisms.
3. Non-Selective Medium:
   • Supports the growth of a broad spectrum of bacteria, making it useful for subculturing and checking the purity of cultures.
4. Customizable Formulation:
   • May be enriched for special-purpose cultivation while retaining its effectiveness as a general-purpose medium.
5. High Yield:
   • One 500g package yields approximately 17.9 liters of prepared medium, ensuring cost-effectiveness and efficiency.

Specifications

Applications

1. Microbial Cultivation:
   • Cultivates non-fastidious bacteria from clinical, food, and environmental samples.
2. Microbial Maintenance:
   • Used for maintaining microorganisms as agar slopes, butts, or plates.
3. Culture Preparation:
   • Subcultures organisms for purity checks or preparation for biochemical and serological testing.
4. Teaching and Research:
   • A reliable medium for laboratory demonstrations, microbiological education, and experiments.
5. Custom Enrichment:
   • Can be enhanced with blood or other biological fluids for the cultivation of specific organisms.

Advantages

1. High Nutrient Availability:
   • Provides a rich source of nutrients for bacterial growth without being overly selective.
2. Flexibility:
   • Adaptable to various microbiological applications by incorporating additional components as needed.
3. Ease of Use:
   • Pre-mixed, dehydrated format simplifies preparation and ensures consistency in results.
4. Cost-Effective:
   • High yield (17.9L per 500g) reduces the cost per sample.
5. Broad Compatibility:
   • Meets multiple international standards, ensuring compatibility with laboratory protocols worldwide.

Preparation Instructions

1. Suspend 28g of the dehydrated powder in 1 liter of distilled or deionized water.
2. Mix thoroughly and heat to boil, shaking frequently to ensure complete dissolution.
3. Sterilize by autoclaving at 121°C for 15 minutes.
4. Cool to approximately 45-50°C before pouring into Petri dishes or using as slopes/butts.

Storage and Stability

• Storage Conditions:
  • Store the powder at 10-30°C in a dry, sealed container to prevent moisture absorption.
• Shelf Life:
  • Retains stability and effectiveness for the duration of its labeled shelf life when stored appropriately.

Intended Use

A general-purpose medium for cultivating non-fastidious microorganisms. It can be enriched with additives such as blood for specialized purposes or used to maintain microorganisms from clinical and other sample types.

Compliance and References

• Certifications: AFNOR, AOAC, BSI, FDA, ISO, NMKL standards.
• Referenced Methods:
  • Lapage et al., Methods in Microbiology, Academic Press, London.

Packaging

• Quantity: 500g powder
• Yield: Prepares approximately 17.9 liters of medium.
• Unit Size: Each container is individually packaged for laboratory use.

Conclusion

The Thermo Scientific™ Oxoid™ Nutrient Agar (Dehydrated, 500g) is a trusted, versatile medium ideal for cultivating a broad range of microorganisms. Its nutrient-rich formula, flexibility for enrichment, and compliance with international standards make it a valuable resource for clinical, research, and teaching laboratories worldwide.