Invitrogen™ One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli, 21 x 50 µL/tube

Invitrogen One Shot™ BL21 Star™ (DE3) Chemically Competent E. coli is an advanced strain optimized for the high-level expression of recombinant proteins. It is particularly suited for low-copy-number, T7-promoter-based systems, offering enhanced mRNA stability and reduced proteolysis.

Key Features:

1. High Protein Expression:
   • Enhanced mRNA stability due to the rne131 mutation, which reduces endogenous RNase activity.
   • Supports T7 RNA polymerase-driven expression, yielding high levels of recombinant protein.
2. Reduced Protein Degradation:
   • Protease Deficiency: Lacks lon and OmpT proteases, minimizing degradation of heterologous proteins.
3. Optimized for Low-Copy Plasmids:
   • Specifically designed for low-copy-number T7-promoter-based plasmids such as Champion™ pET vectors.
4. Single-Tube Transformation:
   • Supplied in the One Shot™ format, allowing transformation and plating in the same tube to save time and prevent contamination.
5. Fast Growth and High Density:
   • Exhibits rapid growth in minimal media and reaches high cell density in glucose-rich media with low acetate production.
6. Inducible Expression System:
   • Expression of the T7 RNA polymerase is controlled by the lacUV5 promoter and induced with IPTG.

Applications:

1. Recombinant Protein Production:
   • High-yield production of non-toxic proteins for research or industrial purposes.
2. T7 Promoter Systems:
   • Supports low-copy-number T7 plasmids like Champion™ pET vectors.
3. RNA Stability Studies:
   • Increased stability of mRNA for higher yields of recombinant protein.

Specifications:

Genotype:

• F– ompT hsdSB (rB–, mB–) gal dcm rne131 (DE3)

Kit Contents:

Advantages:

Handling and Storage:

• Store Competent Cells: –80°C.
• Store pUC19 DNA: –20°C.
• Store S.O.C. Medium: 4°C or room temperature.
• Follow standard laboratory safety procedures during handling.