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Bioss™ Kilham Rat Virus/KRV-VP1/KRV-VP2 Polyclonal Antibody, 100 µL
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Specifications:
| Application | Western Blot, immunocytochemistry, ELISA-Based Assays, Immunohistochemistry | ||
| Storage Temperature | -20°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Bioss Kilham Rat Virus (KRV) VP1/VP2 Polyclonal Antibody is a rabbit polyclonal IgG designed to detect Kilham Rat Virus viral proteins (VP1 and VP2). Raised against a KLH-conjugated synthetic peptide derived from the C-terminal region of KRV VP1/VP2, this antibody is unconjugated and supplied as a liquid preparation at 1 mg/mL.
Validated for use in multiple immunoassays, the antibody supports applications in Western blotting, immunohistochemistry (paraffin and frozen), immunocytochemistry, immunofluorescence, and ELISA. It is affinity purified via Protein A and formulated in a stabilizing buffer with BSA and glycerol for long-term storage.
Key Features
- Specific target – recognizes Kilham Rat Virus VP1/VP2 proteins (C-terminus).
- Versatile applications – validated for WB, IHC (P), IHC (F), ICC/IF, and ELISA.
- High-quality rabbit polyclonal antibody – purified via Protein A for reproducibility.
- Flexible use – suitable for multiple detection platforms and sample types.
- Stabilized liquid format – supplied at 1 mg/mL in TBS with BSA and glycerol.
Applications and Tested Dilutions
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | 1:500 – 1:2,000 |
| Immunohistochemistry (Paraffin) – IHC (P) | Assay-dependent |
| Immunohistochemistry (Frozen) – IHC (F) | 1:100 – 1:500 |
| Immunocytochemistry / IF | 1:50 – 1:200 |
| ELISA | 1:500 – 1:1,000 |
(Optimal dilutions should be determined experimentally.)
Technical Specifications
| Property | Value |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Antibody Type | Polyclonal |
| Conjugate | Unconjugated |
| Concentration | 1 mg/mL |
| Immunogen | KLH-conjugated synthetic peptide (KRV VP1/VP2 C-terminal) |
| Purification Method | Protein A |
| Form | Liquid |
| Storage Buffer | 0.01 M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Preservative | 0.02% ProClin 300 |
| Species Reactivity | Kilham Rat Virus (KRV) |
| Storage Conditions | –20 °C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| Catalog Number | BS-4643R |
| Quantity Available | 100 µL |
Target Information
Kilham Rat Virus (KRV) is a parvovirus identified in rats, with VP1 and VP2 proteins expressed as structural viral components. KRV infection can cause mortality in newborn rats following intracerebral, intravenous, or subcutaneous administration, with viral replication leading to detectable infectious particles and hemagglutinins in multiple tissues, blood, and urine.
The Bioss Kilham Rat Virus VP1/VP2 Polyclonal Antibody provides a reliable and versatile tool for detecting KRV proteins in immunoassays. With broad application coverage, high-quality purification, and stabilized formulation, it is well-suited for researchers investigating parvovirus infection models and host-pathogen interactions.