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Specifications:
| Application | Transfection | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Promega | ||
| Product Grade | Molecular Biology | ||
The Bright-Glo™ Luciferase Assay System is a high-sensitivity, homogeneous firefly luciferase reporter assay designed for continuous-processing robotic platforms and high-throughput screening. Optimized for mammalian cell-based assays, it delivers a bright, stable bioluminescent signal with a half-life of approximately 30 minutes, ensuring precision and reproducibility even in automated workflows.
Unlike conventional luciferase assays, Bright-Glo™ eliminates the need for sample preprocessing—cells can be grown and assayed directly in the same multiwell plate without removing culture medium or washing cells.
Key Features
- No Preprocessing Required – Add reagent directly to cells in culture medium without washing or medium removal.
- High Sensitivity – Up to 10× more sensitive than other homogeneous luciferase reagents.
- Stable Signal – 30-minute half-life supports robotic and batch processing.
- Improved Reproducibility – Reduced sensitivity to mixing conditions, evaporation, and pipetting errors.
- Automation-Ready – Compatible with continuous-process and high-throughput systems.
Applications
- Promoter & Gene Expression Analysis – Quantify firefly luciferase activity in mammalian cells.
- High-Throughput Screening (HTS) – Automation-friendly for large assay runs.
- RNAi & miRNA Studies – Assess post-transcriptional regulation effects.
- Drug Discovery – Screen compounds affecting gene regulation or signaling pathways.
- Pathway Reporter Assays – Study transcriptional and signal transduction activity.
Technical Specifications
| Parameter | Details |
|---|---|
| Detection Method | Bioluminescence |
| Reporter Enzyme | Firefly (Photinus pyralis) luciferase |
| Signal Half-Life | ~30 minutes |
| Assay Type | Homogeneous, no-wash |
| Formats | 10 mL, 100 mL, 10 × 100 mL |
| Sample Types | Mammalian cell cultures |
| Compatibility | Multiwell plates, automation systems |
Kit Components
| Catalog Number | Kit Size | Contents |
|---|---|---|
| E2610 | 10 mL | 1 × Bright-Glo™ Luciferase Assay Substrate (E263A), 1 × 10 mL Bright-Glo™ Luciferase Assay Buffer (E264A) |
| E2620 | 100 mL | 1 × Bright-Glo™ Luciferase Assay Substrate (E263B), 1 × 100 mL Bright-Glo™ Luciferase Assay Buffer (E264B) |
| E2650 | 10 × 100 mL | 10 × Bright-Glo™ Luciferase Assay Substrate (E263B), 10 × 100 mL Bright-Glo™ Luciferase Assay Buffer (E264B) |
The Bright-Glo™ Luciferase Assay System is a top-tier choice for high-sensitivity firefly luciferase detection, whether in small-scale assays or fully automated HTS workflows. Its robust signal stability, ease of use, and compatibility with robotic systems make it an excellent solution for gene expression research, pathway analysis, and drug discovery.
- Pack Size: 100 mL 10 mL 10 x 100 mL