Atom Sci. Eosin Y Certified C.I. 45380

The Atom Scientific Eosin Y Certified is a high-quality, certified grade dye commonly used as a counterstain in the Haematoxylin and Eosin (H&E) staining method. This dark red-brown powder dye plays a critical role in histology and pathology for differentiating cellular structures under the microscope. Its certified grade ensures consistent and reliable results in both research and clinical applications.

Key Features

1. Certified Quality

• C.I. 45380 Certified:
  • Assures compliance with international standards for quality and performance.
  • Ideal for routine and advanced histological staining.

2. Applications

• Counterstain in H&E Staining:
  • Stains cytoplasmic components, extracellular matrix, and connective tissues in shades of pink to red.
  • Complements haematoxylin’s nuclear staining, providing excellent contrast.
• Special Staining Protocols:
  • Suitable for additional histological and pathological staining methods requiring an acidic dye.
• Microscopy and Diagnostics:
  • Widely used for tissue sample preparation in histology, pathology, and diagnostic settings.

3. Physical Properties

• Appearance: Dark red-brown powder.
• Solubility: Readily dissolves in water or ethanol.
• Chemical Class: Acidic xanthene dye.
• Molecular Formula: C20H8Br4O5Na2.

Benefits

• High Contrast Staining:
  • Enhances visualization of cellular and extracellular structures by providing distinct pink-to-red hues.
• Versatile Use:
  • Compatible with manual and automated staining systems.
• Reliable and Reproducible:
  • Certified grade ensures consistency in laboratory workflows.

Preparation and Usage

Preparation:

1. Dissolve Eosin Y powder in distilled water or ethanol to prepare a working solution (typically 0.5–1% concentration).
2. Acidify with a few drops of acetic acid if required for improved staining properties.

Staining Protocol:

1. Following haematoxylin staining, immerse tissue sections in Eosin Y solution for 1–2 minutes.
2. Rinse in water or ethanol to remove excess dye.
3. Observe under a microscope to ensure appropriate staining contrast.

Specifications

Storage and Handling

• Store at room temperature in a dry, sealed container.
• Protect from direct sunlight and moisture.
• Handle with proper personal protective equipment (PPE).

Safety Precautions

• Avoid inhalation, ingestion, or direct contact with skin or eyes.
• Use in a well-ventilated laboratory space.
• Dispose of waste according to local regulations.
• Consult the safety data sheet (SDS) for detailed hazard information.

The Atom Scientific Eosin Y Certified (C.I. 45380) is an essential reagent for histological staining, offering reliable and consistent results for laboratories specializing in H&E staining. Its certified quality ensures it meets the rigorous demands of both research and clinical diagnostics, making it a trusted choice for professionals in histology and pathology.