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Made-to-Order qPCR Assays for Gene Expression Analysis
Applied Biosystems™ Custom TaqMan™ Gene Expression Assays are made-to-order real-time PCR assays designed for quantitative gene expression analysis. These assays provide the custom version of Applied Biosystems™ predesigned TaqMan™ assays and are suitable when a specific target, transcript, gene variant, or sequence region requires a customized assay design.
Each assay includes a pair of unlabeled PCR primers and a sequence-specific TaqMan™ probe labeled with FAM dye at the 5′ end, plus a minor groove binder (MGB) and nonfluorescent quencher (NFQ) at the 3′ end. This chemistry supports high specificity, strong sensitivity, and accurate quantification of gene expression by real-time PCR.
Key Features
| Feature | Description |
|---|---|
| Custom assay design | Made-to-order assay for user-specified gene expression targets |
| FAM-labeled probe | Includes a TaqMan™ MGB probe with FAM dye for real-time fluorescence detection |
| High specificity | MGB probe chemistry improves target specificity and helps reduce cross-reactivity |
| Sensitive detection | Suitable for low-expression genes and low-abundance targets |
| Accurate quantification | Enables reliable detection of small fold-changes in gene expression |
| Easy qPCR setup | Add cDNA and master mix; no melt curve analysis required |
| Pre-formulated assay mix | Supplied as a ready-to-use primer and probe assay mix |
| Sequence information provided | Assay sequences are provided with every custom assay ordered |
Product Variants
| Catalog Number | Assay Size | Quantity | Format |
|---|---|---|---|
| 4331348 | S | 360 reactions | Made to order |
| 4332078 | M | 750 reactions | Made to order |
| 4332079 | L | 2,900 reactions | Made to order |
Applications
| Application Area | Use |
|---|---|
| Gene expression analysis | Quantitative measurement of mRNA expression levels |
| qPCR research workflows | Real-time PCR analysis using TaqMan™ probe-based chemistry |
| Low-abundance target detection | Sensitive quantification of targets expressed at low levels |
| Transcript analysis | Custom assay design for specific gene or transcript targets |
| Gene variant studies | Assay development for targets with high sequence similarity |
| Biomarker research | Quantification of expression changes in research samples |
| Validation studies | Confirmation of expression results from discovery workflows |
Assay Format and Composition
| Component | Description |
|---|---|
| PCR primers | 2 unlabeled PCR primers |
| Probe | 1 FAM dye-labeled TaqMan™ MGB probe |
| Probe quencher | Nonfluorescent quencher, NFQ |
| Probe chemistry | MGB probe for improved specificity |
| Final 1X primer concentration | 900 nM each |
| Final 1X probe concentration | 250 nM |
| Recommended master mix | TaqMan™ Fast Advanced Master Mix |
Compatible Instruments
| Applied Biosystems™ Real-Time PCR Systems |
|---|
| 7500 System |
| 7500 Fast System |
| 7900HT System |
| StepOne™ |
| StepOnePlus™ |
| ViiA™ 7 System |
| QuantStudio™ 3 |
| QuantStudio™ 5 |
| QuantStudio™ 6 Flex |
| QuantStudio™ 7 Flex |
| QuantStudio™ 6 Pro |
| QuantStudio™ 7 Pro |
| QuantStudio™ 12K Flex |
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Applied Biosystems™ Custom TaqMan™ Gene Expression Assay, FAM |
| Product Line | TaqMan™ |
| Product Type | Custom TaqMan™ Gene Expression Assay |
| Detection Dye | FAM |
| Probe Type | TaqMan™ MGB probe |
| Quencher | NFQ, nonfluorescent quencher |
| Assay Format | Pre-formulated primer and probe mix |
| S and M Size Concentration | 20X assay mix |
| L Size Concentration | 60X assay mix |
| Shipping Condition | Room temperature |
| Storage | Store at -15°C to -25°C |
| Unit Size | Each |
| Use Statement | For Research Use Only. Not for use in diagnostic procedures |
Unique Advantages
| Advantage | Benefit |
|---|---|
| Gold-standard TaqMan™ chemistry | Supports reliable probe-based qPCR performance |
| MGB probe design | Allows shorter probes with improved specificity |
| No melt curve requirement | Simplifies workflow compared with dye-based assays |
| Custom target flexibility | Useful when predesigned assays are not available or when a specific sequence is required |
| Bioinformatics-supported design | Helps improve specificity and minimize cross-reactivity |
| Multiple reaction sizes | Enables users to select an assay size based on project scale |
Applied Biosystems™ Custom TaqMan™ Gene Expression Assays, FAM provide reliable made-to-order qPCR assays for quantitative gene expression research. With FAM-labeled TaqMan™ MGB probe chemistry, pre-formulated primers and probe, and compatibility with a wide range of Applied Biosystems™ real-time PCR systems, these assays are well suited for sensitive, specific, and accurate measurement of gene expression targets.
Brochures & Specifications: TaqMan Gene Expression Assays Brochure
Manual: Introduction to Gene Expression Getting Started Guide
Protocol: Custom TaqMan® Assays Design and Ordering for SNP Genotyping and Gene Expression Assays
User Guide: TaqMan Multiplex PCR Optimization
Made-to-Order qPCR Assays for Gene Expression Analysis
Applied Biosystems™ Custom TaqMan™ Gene Expression Assays are made-to-order real-time PCR assays designed for quantitative gene expression analysis. These assays provide the custom version of Applied Biosystems™ predesigned TaqMan™ assays and are suitable when a specific target, transcript, gene variant, or sequence region requires a customized assay design.
Each assay includes a pair of unlabeled PCR primers and a sequence-specific TaqMan™ probe labeled with FAM dye at the 5′ end, plus a minor groove binder (MGB) and nonfluorescent quencher (NFQ) at the 3′ end. This chemistry supports high specificity, strong sensitivity, and accurate quantification of gene expression by real-time PCR.
Key Features
| Feature | Description |
|---|---|
| Custom assay design | Made-to-order assay for user-specified gene expression targets |
| FAM-labeled probe | Includes a TaqMan™ MGB probe with FAM dye for real-time fluorescence detection |
| High specificity | MGB probe chemistry improves target specificity and helps reduce cross-reactivity |
| Sensitive detection | Suitable for low-expression genes and low-abundance targets |
| Accurate quantification | Enables reliable detection of small fold-changes in gene expression |
| Easy qPCR setup | Add cDNA and master mix; no melt curve analysis required |
| Pre-formulated assay mix | Supplied as a ready-to-use primer and probe assay mix |
| Sequence information provided | Assay sequences are provided with every custom assay ordered |
Product Variants
| Catalog Number | Assay Size | Quantity | Format |
|---|---|---|---|
| 4331348 | S | 360 reactions | Made to order |
| 4332078 | M | 750 reactions | Made to order |
| 4332079 | L | 2,900 reactions | Made to order |
Applications
| Application Area | Use |
|---|---|
| Gene expression analysis | Quantitative measurement of mRNA expression levels |
| qPCR research workflows | Real-time PCR analysis using TaqMan™ probe-based chemistry |
| Low-abundance target detection | Sensitive quantification of targets expressed at low levels |
| Transcript analysis | Custom assay design for specific gene or transcript targets |
| Gene variant studies | Assay development for targets with high sequence similarity |
| Biomarker research | Quantification of expression changes in research samples |
| Validation studies | Confirmation of expression results from discovery workflows |
Assay Format and Composition
| Component | Description |
|---|---|
| PCR primers | 2 unlabeled PCR primers |
| Probe | 1 FAM dye-labeled TaqMan™ MGB probe |
| Probe quencher | Nonfluorescent quencher, NFQ |
| Probe chemistry | MGB probe for improved specificity |
| Final 1X primer concentration | 900 nM each |
| Final 1X probe concentration | 250 nM |
| Recommended master mix | TaqMan™ Fast Advanced Master Mix |
Compatible Instruments
| Applied Biosystems™ Real-Time PCR Systems |
|---|
| 7500 System |
| 7500 Fast System |
| 7900HT System |
| StepOne™ |
| StepOnePlus™ |
| ViiA™ 7 System |
| QuantStudio™ 3 |
| QuantStudio™ 5 |
| QuantStudio™ 6 Flex |
| QuantStudio™ 7 Flex |
| QuantStudio™ 6 Pro |
| QuantStudio™ 7 Pro |
| QuantStudio™ 12K Flex |
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Applied Biosystems™ Custom TaqMan™ Gene Expression Assay, FAM |
| Product Line | TaqMan™ |
| Product Type | Custom TaqMan™ Gene Expression Assay |
| Detection Dye | FAM |
| Probe Type | TaqMan™ MGB probe |
| Quencher | NFQ, nonfluorescent quencher |
| Assay Format | Pre-formulated primer and probe mix |
| S and M Size Concentration | 20X assay mix |
| L Size Concentration | 60X assay mix |
| Shipping Condition | Room temperature |
| Storage | Store at -15°C to -25°C |
| Unit Size | Each |
| Use Statement | For Research Use Only. Not for use in diagnostic procedures |
Unique Advantages
| Advantage | Benefit |
|---|---|
| Gold-standard TaqMan™ chemistry | Supports reliable probe-based qPCR performance |
| MGB probe design | Allows shorter probes with improved specificity |
| No melt curve requirement | Simplifies workflow compared with dye-based assays |
| Custom target flexibility | Useful when predesigned assays are not available or when a specific sequence is required |
| Bioinformatics-supported design | Helps improve specificity and minimize cross-reactivity |
| Multiple reaction sizes | Enables users to select an assay size based on project scale |
Applied Biosystems™ Custom TaqMan™ Gene Expression Assays, FAM provide reliable made-to-order qPCR assays for quantitative gene expression research. With FAM-labeled TaqMan™ MGB probe chemistry, pre-formulated primers and probe, and compatibility with a wide range of Applied Biosystems™ real-time PCR systems, these assays are well suited for sensitive, specific, and accurate measurement of gene expression targets.
Brochures & Specifications: TaqMan Gene Expression Assays Brochure
Manual: Introduction to Gene Expression Getting Started Guide
Protocol: Custom TaqMan® Assays Design and Ordering for SNP Genotyping and Gene Expression Assays
User Guide: TaqMan Multiplex PCR Optimization
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Specifiche
| Reactions | 360 reactions, 750 reactions, 2,900 reactions |