Applied Biosystems™ TaqMan™ TAMRA Probe

The TaqMan™ TAMRA Probe is a dual-labeled probe specifically designed for real-time PCR (qPCR) using TaqMan chemistry. It incorporates a 5′ fluorescent reporter dye (FAM, VIC, or TET) and a 3′ quencher dye (TAMRA) for accurate and sensitive DNA quantification and analysis. TAMRA probes were among the pioneering TaqMan probes, supporting robust qPCR performance across various applications.

Key Features:

1. Dual-Labeled Fluorescent Probe:
   • 5′ Reporter Dyes: FAM, VIC, or TET, enabling the detection of target sequences.
   • 3′ Quencher Dye: TAMRA (Tetramethylrhodamine), which quenches fluorescence in proximity to the reporter.
2. Broad Multiplexing Capability:
   • Suitable for up to three targets in multiplexed qPCR experiments.
   • For higher multiplexing, consider TaqMan QSY probes.
3. Length and Specificity:
   • Probes are typically longer (30 to >40 bases) for enhanced hybridization stability.
   • For applications requiring shorter probes (15–20 bases), TaqMan MGB NFQ probes offer improved specificity.
4. High Purity and Reproducibility:
   • Purified using HPLC (High-Performance Liquid Chromatography).
   • Verified by mass spectrometry to ensure high-quality and lot-to-lot consistency.
5. Flexibility:
   • Available in liquid or dried format.
   • Customizable for specific needs with various dyes, concentrations, and lot sizes via the TaqMan Custom Assay and Oligo Service.
6. Guaranteed Yield and Shipping:
   • Minimum guaranteed yield for selected unit sizes (6,000 pmol, 20,000 pmol, or 50,000 pmol).

Applications:

• Quantitative PCR (qPCR): Accurate quantification of DNA or RNA targets.
• Gene Expression Studies: Multiplexed detection and quantification of multiple genes in a single run.
• Mutation Detection: Differentiation of wild-type and mutant sequences.
• Copy Number Variation (CNV) Analysis: Precise measurement of gene copy numbers.

Technical Specifications:

Instrument Compatibility:

• Compatible with the following real-time PCR systems:
  • QuantStudio™ Systems: 3, 5, 6 Flex, 7 Flex, 6 Pro, 7 Pro, 12k Flex.
  • ViiA™ 7 System.
  • StepOne™ and StepOnePlus™ Systems.
  • 7900HT and 7500/7500 Fast Systems.

Advantages Over Alternatives:

1. Versatile and Reliable:
   • Established technology with robust performance in qPCR applications.
2. Customizable Probes:
   • Tailored for specific research needs, including dye options and concentrations.
3. Validated and Certified:
   • Manufactured in an ISO 13485-certified facility, ensuring quality and reproducibility.
4. Sustainability:
   • Environmentally friendly packaging.

References:

• Thermo Fisher Scientific: Product details and specifications.
• TaqMan Chemistry Overview: Applied Biosystems qPCR workflows.