Cholesterol (Standard) is a high-purity analytical reference standard (99.91%) used for quantitative and qualitative analysis in chromatographic and biochemical research applications. This compound is commonly employed as a calibration standard for cholesterol analysis in techniques such as HPLC, GC, and mass spectrometry (MS).

Cholesterol is the major sterol in mammals and represents approximately 20–25% of the lipid composition of the plasma membrane, where it plays a crucial role in maintaining membrane fluidity, permeability, and structural integrity. By regulating the physical properties of cellular membranes, cholesterol influences the activity of membrane transporters, receptors, and signaling proteins.

In addition to its structural functions, cholesterol also acts as an endogenous agonist of estrogen-related receptor alpha (ERRα), making it relevant in studies related to metabolism, nuclear receptor signaling, and cellular energy regulation.

Supplied as a certified analytical reference compound, this product is ideal for method development, calibration, and validation of lipid analysis methods in pharmaceutical, biomedical, and biochemical research laboratories.

For research use only. Not for therapeutic or diagnostic applications.

Key Features

• Ultra-High Purity: 99.91% analytical reference standard
• Suitable for Chromatographic Analysis: HPLC, GC, and LC-MS
• Reliable Calibration Standard for Lipid Analysis
• Essential Sterol Compound in membrane biology research
• Supports Method Development and Validation in analytical laboratories
• Consistent Quality for lipidomics and metabolomics studies

Applications

Cholesterol (Standard) is widely used in:

• HPLC calibration and lipid quantification
• GC and LC-MS cholesterol analysis
• Lipidomics and metabolomics research
• Cell membrane structure studies
• Steroid metabolism research
• Cardiovascular and metabolic disease research
• Pharmaceutical quality control
• Food and nutraceutical lipid analysis
• Biochemical pathway investigations

Chemical and Physical Properties

Biological Significance

Cholesterol is a fundamental lipid molecule involved in multiple biological processes:

• Maintains cell membrane fluidity and stability
• Regulates membrane permeability
• Supports function of membrane transport proteins
• Precursor for steroid hormones
• Precursor for bile acids and vitamin D
• Activator of estrogen-related receptor α (ERRα)

These functions make cholesterol an important molecule in metabolic, cardiovascular, and cellular signaling research.

Ordering Information

Cholesterol (Standard) – 99.91% is a high-quality analytical reference compound widely used in lipid analysis, chromatographic calibration, and biochemical research. Its exceptional purity and well-characterized sterol structure make it an ideal standard for HPLC, GC, and mass spectrometry applications. As a key molecule in membrane biology and lipid metabolism, this compound is essential for lipidomics, metabolomics, pharmaceutical research, and metabolic disease studies.