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Specifications:
| Application | Gel Electrophoresis | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Buffer | Forms | Powder |
| Product Brand | Apollo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₈H₂₀NO₅Cl |
Bis-TRIS hydrochloride is a zwitterionic biological buffer with a useful pH range of 5.8–7.2, making it ideal for biological, biochemical, and diagnostic applications. It is widely used in Phos-tag SDS-PAGE and other electrophoretic techniques for the resolution of phosphorylated proteins under neutral pH conditions. This buffer ensures minimal protein denaturation and excellent resolution in phosphorylation analysis.
Key Features
- High purity (≥99%) for consistent and reproducible results
- Ideal for buffering systems in biochemical assays and diagnostics
- Excellent solubility in water: 0.5 g/mL (clear to faint yellow solution)
- Stable at room temperature; easy storage and handling
- pKa value of 6.5, optimal for near-neutral pH studies
- Used in Phos-tag SDS-PAGE for high-resolution protein phosphorylation analysis
Chemical & Physical Properties
| Property | Specification |
|---|---|
| Chemical Name | Bis-TRIS hydrochloride |
| CAS Number | 124763-51-5 |
| Molecular Formula | C₈H₁₉NO₅·HCl |
| Molecular Weight | 245.70 g/mol |
| Purity (Assay) | ≥99.0% (titration) |
| Form | Crystalline powder |
| Useful pH Range | 5.8–7.2 |
| pKa | 6.5 |
| Solubility | 0.5 g/mL in water (clear, colorless–faint yellow) |
| Storage Temperature | Room temperature |
| MDL Number | MFCD00171252 |
| UNSPSC Code | 12161700 |
| NACRES | NA.25 |
Applications
- Diagnostic assay manufacturing
- Protein electrophoresis (e.g., Phos-tag SDS-PAGE for phosphorylation profiling)
- Buffering biological samples near neutral pH
- Cellular signaling studies involving phosphorylated proteins
- Biochemical research requiring zwitterionic buffering agents
Example Application:
As referenced in research by Kinoshita et al. (2012), Bis-TRIS hydrochloride is effectively used in Phos-tag SDS-PAGE systems to resolve phosphorylated proteins under near-neutral pH, supporting detailed phosphorylation analysis for insights into cellular signaling pathways.
Apollo Scientific Bis-TRIS Hydrochloride (≥98%) is a high-quality buffering agent tailored for diagnostic assays, protein research, and electrophoretic applications. Its stability, neutral pH range, and compatibility with phosphorylation studies make it a versatile and dependable reagent in any molecular biology or biochemical lab.
- Pack Size: 250g 100g