Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

Amicon® Ultra 15 mL centrifugal devices with Ultracel® 10,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.

Application

• Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane.
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
• Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
• Concentration of dilute or pre-purified proteins from column eluents.
• Desalting, buffer exchange and protein dialysis.

Features and Benefits

• Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%.
• Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10-15 minutes).
• Heat-sealed membrane minimizes downstream extractables.
• 100% integrity tested for reliable performance.
• Convenient sample monitoring with translucent housing and volume gradations.
• Direct pipettor sample access eliminates processing step to recover concentrate.
• High concentration factors of 80–100X.

Physical form

Color Code: Clear with pink cap

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, GermanyULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany