Invitrogen™ mirVana™ PARIS™ RNA and Native Protein Purification Kit, 40 preps

The mirVana™ PARIS™ Kit is for the purification of both native protein and RNA (including small RNA) from the same sample. Each kit contains sufficient reagents and consumables for either 40 isolations of protein and total RNA (including small RNAs), or 20 isolations of protein and separate large and small RNA fractions. Features of this kit:

• Isolate small RNA-containing total RNA and native protein from the same sample

• Start with 100–107 cultured cells or up to 100 mg of many mammalian tissues

• Enrich for small RNA (<200 nt) to increase sensitivity in downstream analyses

• Follow a simple, 30-minute procedure

• Use to correlate mRNA, miRNA or siRNA, and protein levels

How the kit works

The mirVana™ PARIS™ Kit procedure begins with homogenization of samples with a special cell disruption buffer that includes nonionic detergent. Protein remains intact, so a portion of the lysate can be used directly for common protein analysis applications. The remainder of the lysate is used for RNA isolation, using a procedure that combines the advantages of organic and solid-phase extraction, while avoiding the disadvantages of both.

Accessory products

For tissues that have very high levels of ribonucleases, such as pancreas or spleen, the mirVana™ miRNA Isolation Kit is recommended, although it is not designed to recover protein and small RNA from the same sample.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Isolation Technology: Organic Extraction, Cell Lysis (Nonionic Detergent), Spin Column (Glass Fiber Filter)
• Purification Time: 30 min.
• Sample Type: Cell Cultures, Tissue (Mammalian)
• Final Product Type: Total RNA, Transcriptome RNA, siRNA, Protein (Native), snRNA, micro RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Western Blotting, microRNA Analysis, Northern Blotting, cDNA Library Construction
• No. of Reactions: 40 Preps
• Product Line: mirVana™, Ambion™
• Shipping Condition: Room Temperature
• Starting Material Amount: Up to 10^7 cells, Up to 100 mg tissue
• Target: Total RNA, RNA and Protein, siRNA, miRNA

Contents & Storage

• 30 mL miRNA Wash Solution I (room temperature)
• 50 mL Wash Solution 2/3 (room temperature)
• 80 Collection Tubes (room temperature)
• 40 Filter Cartridges (room temperature)
• 25 mL Cell Disruption Buffer (4°C)
• 25 mL 2X Denaturing Solution (4°C)
• 2 × 25 mL Acid-Phenol:Chloroform (4°C)
• 5 mL Elution Solution (-20°C, 4°C, or room temperature)