Applied Biosystems™ VetMAX™ African Swine Fever Virus Detection Kit 2.0

The Applied Biosystems™ VetMAX™ African Swine Fever Virus Detection Kit 2.0 is a highly sensitive, second-generation real-time PCR (qPCR) assay designed for the rapid and reliable detection of African Swine Fever Virus (ASFV) DNA in porcine and wild species samples.

This advanced, single-well multiplex assay simultaneously amplifies the ASFV target along with dual internal positive controls (IPC)—both endogenous and exogenous—using TaqMan™ probe-based fluorescence detection. This ensures accurate, validated results while monitoring sample integrity and amplification efficiency.

The VetMAX ASFV Detection Kit 2.0 enables laboratories to perform fast, robust, and high-confidence screening for ASFV in outbreak investigations, surveillance programs, and veterinary diagnostic workflows.

Key Features

• Single-Well Multiplex Detection
  Simultaneous amplification and detection of ASFV DNA and dual internal controls.
• Dual Internal Positive Controls (IPC)
  Includes endogenous IPC (sample integrity control) and Xeno™ exogenous IPC (process control).
• Ready-to-Use Master Mix
  Simplifies workflow, minimizes pipetting errors, and improves reproducibility.
• Rapid qPCR Detection
  Delivers results in approximately 30–50 minutes.
• High Sensitivity and Specificity
  Demonstrated performance with 100% sensitivity and 100% specificity.
• Pooling Capability
  Allows pooling of up to 20 samples, improving throughput and reducing testing costs.
• Compatible with Fast MagMAX CORE Extraction Protocol
  Enables sample preparation in approximately 20 minutes.
• Validated for Multiple Sample Types
  Suitable for blood, serum, tissues, organs, oral fluids, and meat juice.

Applications

• Veterinary diagnostic laboratories
• National veterinary surveillance programs
• Veterinary research institutions
• Livestock disease monitoring programs
• Outbreak investigation and control
• Veterinary reference laboratories
• Agricultural and food safety laboratories

Target Pathogen Information

African Swine Fever Virus (ASFV) is a highly contagious, lethal viral disease affecting domestic pigs and wild boars. Rapid and accurate detection is critical for controlling outbreaks and minimizing economic losses in pig-producing regions.

The VetMAX ASFV Detection Kit enables reliable identification of infected animals, supporting early containment and biosecurity measures.

Technical Specifications

Workflow Overview

Sample Preparation:

MagMAX CORE protocol (~20 minutes)

PCR Amplification:

Real-time PCR run (~30–50 minutes)

Total Detection Time:

Under 1 hour

Compatible Instruments

The VetMAX ASFV Detection Kit 2.0 is compatible with Applied Biosystems real-time PCR systems, including:

• Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System
• Applied Biosystems™ QuantStudio™ 7 Real-Time PCR System
• Applied Biosystems™ 7500 Fast Real-Time PCR System
• Applied Biosystems™ StepOnePlus™ Real-Time PCR System

Advantages for Veterinary Diagnostic Laboratories

• Rapid outbreak detection
• High confidence diagnostic accuracy
• Reduced workflow time
• Lower testing cost through sample pooling
• Robust assay performance with internal validation controls
• Ready-to-use format simplifies workflow

Ordering Information

The Applied Biosystems™ VetMAX™ African Swine Fever Virus Detection Kit 2.0 provides a fast, reliable, and highly sensitive qPCR solution for detecting ASFV in veterinary diagnostic applications. With multiplex detection, dual internal controls, and rapid turnaround time, it is an essential tool for veterinary laboratories and disease surveillance programs to support timely outbreak response and biosecurity measures.