Applied Biosystems™ MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit, with bead plate, for 100 preps

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Specifications:

Application	Genomic Purification
Storage Temperature	2-8°C
Product Type	Sample Prep
Product Brand	Thermo Fisher Scientific™
Product Grade	Molecular Biology

Overview
Resource

The MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit is a cutting-edge solution for high-throughput RNA and DNA extraction from challenging sample types such as stool and soil. The kit leverages MagMAX magnetic-bead technology for efficient, reproducible, and high-yield nucleic acid recovery, tailored for applications like real-time PCR, NGS, and microarrays.

Key Features

Automation-Ready Protocols:
- Designed for use with KingFisher™ Flex, KingFisher™ Duo Prime, and KingFisher™ Apex systems for seamless high-throughput processing.
- Process up to 96 samples in ∼60 minutes with KingFisher Flex.
Broad Sample Compatibility:
- Suitable for nucleic acid extraction from stool, soil, biofluids, swabs (buccal or skin), and yeast.
- Accommodates up to 100 mg stool or 250 mg soil per well.
Dual Bead Beating Formats:
- High-throughput 96-deep well plates or individual tube formats for lower throughput.
High-Quality Magnetic Beads:
- Dynabeads MyOne Silane offers superior binding efficiency for RNA and DNA recovery.
- Reduces risk of filter clogging, especially for stool samples.
Flexible Elution Volumes:
- Recover nucleic acids in 50–200 μL of elution buffer for downstream applications.
High Throughput & Versatile Applications:
- Compatible with RT-PCR, qPCR, NGS, and hybridization-based applications.
- High-throughput capability ideal for microbiome research and pathogen detection.

Specifications

Feature	Details
Isolation Technology	Magnetic Bead
Sample Compatibility	Stool, Soil, Biofluids, Swabs (Buccal/Skin), Yeast
Final Product Type	RNA and DNA
Elution Volume	50–200 μL
Applications	RT-PCR, qPCR, NGS, microarray analysis
Processing Time	∼60 minutes (96 samples, KingFisher Flex)
Starting Material Amount	Stool: ≤100 mg; Soil: ≤250 mg; Biofluids/Yeast: ≤500 μL
High-Throughput Compatibility	Yes
Unit Size	100 preps
Shipping Condition	Room Temperature

Kit Contents

Component	Quantity	Storage
Lysis Buffer	80 mL	Room Temperature
Binding Solution	50 mL	Room Temperature
Wash Buffer	200 mL	Room Temperature
Elution Solution	20 mL	Room Temperature
Proteinase K	4 mL	Room Temperature
Total Nucleic Acid Binding Beads	2 mL	Room Temperature
96-Deep Well Plate	1 Plate	Room Temperature

Comparison with Competitor Products

Feature	MagMAX Microbiome Kit	Qiagen PowerSoil DNA Kit	Zymo Biomics DNA Miniprep Kit
Technology	Magnetic Bead	Bead Beating	Bead Beating
High-Throughput Compatibility	Yes (96 samples in ∼60 min with KingFisher)	No	Yes
Sample Compatibility	Stool, Soil, Biofluids, Swabs, Yeast	Stool, Soil	Stool, Soil, Saliva, Swabs
Starting Material	Stool: ≤100 mg; Soil: ≤250 mg	Soil: ≤250 mg	Stool: ≤200 mg
Elution Volume	50–200 μL	100 μL	50–200 μL
Output Nucleic Acid	RNA and DNA	DNA only	DNA only
Processing Time	∼60 minutes for 96 samples (KingFisher Flex)	∼60 minutes per batch	∼60 minutes
Applications	RT-PCR, NGS, Microarray, Hybridization	qPCR, Sequencing	Sequencing, PCR

Advantages of MagMAX Microbiome Kit

Higher Efficiency: Magnetic beads outperform traditional glass-fiber filters, ensuring higher yields and preventing filter clogging in complex samples like stool.
Automation-Ready: Seamless integration with KingFisher systems for high-throughput applications.
Dual Nucleic Acid Isolation: Extract both RNA and DNA in one workflow, providing flexibility for microbiome studies and pathogen detection.
Faster Processing: Streamlined protocols enable rapid nucleic acid extraction from diverse sample types.

Applications

Microbiome Research:
- Analyze microbial communities in stool, soil, and swabs for health and ecological studies.
Pathogen Detection:
- Identify and quantify pathogenic bacteria in clinical and environmental samples.
NGS and Hybridization:
- Use high-quality RNA and DNA for next-generation sequencing or hybridization-based assays.
Genomic Studies:
- Study microbial genomes in various environmental and biological contexts.

The MagMAX™ Microbiome Ultra Nucleic Acid Isolation Kit stands out as a powerful and versatile solution for researchers working with challenging microbiome samples, offering speed, efficiency, and compatibility with high-throughput platforms.