Thermo Scientific™ Pierce™ DTT (Dithiothreitol), No-Weigh™ Format, 48 x 7.7 mg

Thermo Scientific™ Pierce™ DTT (Dithiothreitol) in No-Weigh™ format is a highly convenient reducing agent designed to simplify protein sample preparation. Supplied in pre-aliquoted, lyophilized 7.7 mg microtubes, this reagent enables rapid preparation of fresh 500 mM DTT stock solutions by simply adding 100 µL of water or buffer.

DTT is a thiol-based reducing agent widely used in molecular biology, biochemistry, and proteomics to break disulfide bonds in proteins and maintain free sulfhydryl (-SH) groups. It is essential in SDS-PAGE, mass spectrometry, protein labeling, and enzyme preservation workflows. However, because DTT solutions degrade rapidly, this single-use format ensures full reducing strength and eliminates waste or variability associated with bulk storage.

Key Features & Benefits

• Ready-to-Use Microtubes
  – Pre-weighed 7.7 mg DTT in each tube yields 100 µL of 500 mM stock; no weighing required.
• Ensures Fresh Reducing Strength
  – Eliminates the need to store unstable DTT solutions; prepares fresh solution on demand.
• Saves Time & Reduces Waste
  – Prepare just the right amount for each experiment—perfect for low-volume or sensitive workflows.
• Precise & Reproducible
  – Each tube is batch-controlled for quality and consistency.
• Fast & Simple Preparation
  – Dissolve in 30 seconds with water or buffer.

Applications

• Protein Sample Preparation
  – Reduction of disulfide bonds in proteins for electrophoresis (SDS-PAGE) or Western blotting.
• Mass Spectrometry
  – Prepares samples for accurate peptide and protein mass analysis.
• Thiol Labeling
  – Reduction prior to sulfhydryl-specific labeling reactions.
• Protein Stabilization
  – Prevents oxidation of cysteine residues and maintains enzyme activity.

Chemical Properties

Thermo Scientific™ Pierce™ DTT (No-Weigh™ Format) is the ideal solution for labs seeking consistent, high-purity reducing agent preparations without the hassle of weighing or waste. Its single-use format ensures that each experiment benefits from maximum reducing efficiency, making it perfect for demanding protein research and analytical applications.