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Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 96 reactions, 0.1 mL
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Specifications:
| Application | qPCR | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | PCR Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
Multiplex real-time PCR kit for simultaneous detection of Zika, Dengue, and Chikungunya virus RNA in urine and serum
The Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit is a lyophilized, multiplex, one-step real-time PCR assay designed for the simultaneous detection and differentiation of three clinically important arboviruses—Zika virus (ZIKV), Dengue virus (DENV1–4), and Chikungunya virus (CHIKV)—directly from human urine or serum samples.
Optimized for use with the Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System, this kit is also compatible with the 7500, 7500 Fast, and other QuantStudio systems. It includes a human PPIA (cyclophilin A) primer-probe set to serve as an endogenous extraction and amplification control, ensuring the integrity and reliability of assay results.
The assay’s lyophilized format eliminates the need for cold chain management, enabling room temperature shipping and storage, making it ideal for field use and resource-limited settings.
Key Features & Benefits
- Triple Target Detection – Detect and differentiate ZIKV, DENV (all 4 serotypes), and CHIKV in a single reaction.
- Lyophilized One-Step RT-PCR – Master mix and primers are pre-formulated; simply add RNA.
- Endogenous Control – Human PPIA ensures proper extraction and RT-PCR performance monitoring.
- Environmentally Friendly & Convenient – No refrigeration required; stable at room temperature.
- Broad Instrument Compatibility – Validated for QuantStudio™ 5, 7500, 7500 Fast, and other QuantStudio systems.
- Rapid & Reliable – Supports both fast and standard cycling protocols for flexible workflows.
Applications
- Multiplex detection of arboviruses in human clinical samples
- Public health surveillance and outbreak monitoring
- Arbovirus diagnostic research and assay validation
- Laboratory preparedness for vector-borne disease detection
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Zika Virus Triplex Kit |
| Product Line | TaqMan™ |
| Format | Tube |
| Form | Lyophilized |
| No. of Reactions | 96 |
| Volume | 0.1 mL |
| PCR Method | 1-step RT-qPCR |
| Reaction Speed | Fast or Standard |
| Detection Method | Primer-probe |
| Probe Reporter Dyes | FAM (Zika), VIC (Dengue), ABY (Chikungunya), JUN (PPIA control) |
| GC-Rich PCR Performance | Low |
| For Use With | QuantStudio™ 5, 7500, 7500 Fast, other QuantStudio systems |
| Target Pathogens | ZIKV, DENV1–4, CHIKV |
| Control Gene | Human PPIA (cyclophilin A) |
| Sample Types | RNA from human urine or serum |
| Compatible Extraction Kit | MagMAX™ Pathogen RNA/DNA Kit |
| Sufficient For | 96 reactions |
| Shelf Life | As per manufacturer’s instructions |
| Shipping Condition | Room temperature |
| Storage | Room temperature |
| Catalog Number | A31747 |
Contents
- 12 × 8-well strip tubes (96 total reactions)
- Primer-probe mixes for ZIKV, DENV, CHIKV, and human PPIA
- Lyophilized one-step RT-PCR master mix
The Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 0.1 mL offers an efficient, multiplexed, and field-ready solution for detecting three major arboviruses in human urine and serum. With its lyophilized one-step RT-PCR format, built-in endogenous control, and room-temperature stability, it streamlines laboratory workflows while maintaining high sensitivity and specificity. This makes it an invaluable tool for clinical research, epidemiological surveillance, and laboratory readiness against vector-borne viral threats.