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Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 96 Reactions, 0.2 mL
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Specifications:
| Application | qPCR | ||
| Storage Temperature | Room Temperature | ||
| Product Type | PCR Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
Multiplex real-time PCR kit for simultaneous detection of Zika, Dengue, and Chikungunya virus RNA in urine and serum
The Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit is a lyophilized, multiplex, one-step real-time PCR assay designed for the simultaneous detection and differentiation of Zika virus (ZIKV), Dengue virus (DENV1–4), and Chikungunya virus (CHIKV) RNA from human urine or serum samples.
Optimized for use with the Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System, this kit is also compatible with the 7500 and other QuantStudio platforms. It includes a human PPIA (cyclophilin A) primer-probe set to serve as an endogenous extraction, reverse transcription, and amplification control, ensuring assay integrity and result reliability.
The lyophilized format eliminates the need for cold chain management, allowing room temperature shipping and storage, making it ideal for both laboratory and field-based testing environments.
Key Features & Benefits
- Triple Pathogen Detection – Simultaneous detection and differentiation of ZIKV, DENV (all 4 serotypes), and CHIKV in one assay.
- One-Step Lyophilized RT-PCR – Pre-formulated master mix; just add RNA to start.
- Endogenous Control – Human PPIA for verifying extraction and amplification efficiency.
- Room Temperature Stability – Ships and stores without refrigeration for easier logistics.
- Instrument Flexibility – Compatible with QuantStudio™ 5, 7500, and other qPCR platforms.
- User-Friendly Workflow – Supports both standard and fast qPCR protocols.
Applications
- Multiplex detection of arboviruses in clinical urine and serum samples
- Public health surveillance and outbreak response
- Vector-borne disease research and assay development
- Laboratory training and diagnostic preparedness
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Zika Virus Triplex Kit |
| Product Line | TaqMan™ |
| Format | Tube |
| Form | Lyophilized |
| Volume | 0.2 mL |
| PCR Method | 1-step RT-qPCR |
| Detection Method | Primer-probe |
| Probe Reporter Dyes | FAM (Zika), VIC (Dengue), ABY (Chikungunya), JUN (PPIA control) |
| Sample Types | RNA from human urine or serum |
| Control Gene | Human PPIA (cyclophilin A) |
| Compatible Extraction Kit | MagMAX™ Pathogen RNA/DNA Kit |
| For Use With | QuantStudio™ 5, 7500, other QuantStudio systems |
| Reaction Speed | Fast or Standard |
| Sufficient For | 96 reactions |
| Tube Format | Standard strip (12 × 8-well, 0.2 mL) |
| Shelf Life | As per manufacturer’s guidelines |
| Shipping Condition | Room temperature |
| Storage | Room temperature |
| Catalog Number | A31746 |
Contents
- 12 × 8-well strip tubes (96 total reactions)
- Pre-formulated lyophilized master mix with primers and probes for ZIKV, DENV, CHIKV, and human PPIA control
The Applied Biosystems™ TaqMan™ Arbovirus Triplex Kit (ZIKV/DENV/CHIKV), 0.2 mL delivers a robust, multiplexed solution for rapid detection of three high-priority arboviruses in human urine and serum. With its one-step lyophilized RT-PCR format, built-in endogenous control, and room-temperature stability, it offers unmatched convenience for laboratory and field applications. Designed for accuracy, speed, and operational ease, it is an essential tool for molecular diagnostics research, public health surveillance, and outbreak response.