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Applied Biosystems™ MagMAX™ mirVana™ Total RNA Isolation Kit, 96 preps
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Specifications:
| Application | Genomic Purification | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The MagMAX™ mirVana™ Total RNA Isolation Kit is a comprehensive, phenol-free RNA extraction solution optimized for the isolation of total RNA, including small RNA species such as miRNA, siRNA, and non-coding RNA, from a wide range of biological sample types. Leveraging magnetic bead–based purification, this kit provides high yields of pure, intact RNA suitable for sensitive downstream applications including RT-qPCR, next-generation sequencing (NGS), cDNA library preparation, and microarray analysis.
Designed for both manual and automated processing, the kit seamlessly integrates with KingFisher™ Duo Prime, KingFisher™ Flex, and KingFisher™ Apex magnetic particle processors, supporting flexible throughput—from small batches to 96 samples in a single run. It is especially useful for researchers requiring high-quality microRNA in addition to total RNA, eliminating the need for multiple kits.
Key Features
True Total RNA Recovery—Including Small RNAs
- Isolates full-spectrum RNA: microRNAs (miRNA), siRNA, long non-coding RNA, mRNA, and rRNA.
- Compatible with small and challenging RNA species critical for transcriptomic and regulatory studies.
Broad Sample Compatibility
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Validated for:
- Serum/plasma (≤100 µL)
- Whole blood (≤50 µL)
- Tissue (≤50 mg)
- Urine (≤250 µL)
- Cultured cells (≤10⁶ cells)
Automation-Ready and Scalable
- Easily scaled from 6 to 96 samples.
- Fully compatible with KingFisher™ magnetic particle processors for walkaway automation or can be run manually using a magnetic stand.
Phenol-Free, Streamlined Protocol
- Safer and cleaner protocol with no organic solvents, simplifying waste disposal and enhancing lab safety.
- Reduced hands-on time compared to traditional column- or phenol-based RNA extraction methods.
High Yield and Purity
- Efficient binding of RNA using MagMAX™ magnetic beads improves yield consistency.
- TURBO™ DNase treatment eliminates contaminating DNA for cleaner downstream analysis.
- Avoids clogging problems associated with column filters—especially important for viscous or particulate samples.
Specifications
| Attribute | Details |
|---|---|
| Isolation Technology | Magnetic Bead (MagMAX™) |
| Purification Time | Up to 1.5 hours |
| Elution Volume | 20 to 100 µL |
| Target Molecules | Total RNA, miRNA, siRNA, mRNA, lncRNA |
| Sample Types | Cells, Plasma, Serum, Tissue, Urine, Whole Blood |
| Final Product Type | Total RNA (including small RNA) |
| Automation Compatibility | KingFisher™ Duo Prime, Flex, Apex |
| Throughput | Manual or automated processing (up to 96 samples/run) |
| For Use With Applications | RT-PCR, qPCR, NGS, cDNA library construction, microarray, blotting, etc. |
| No. of Reactions | 96 Preps |
| Shipping Conditions | Room Temperature (Box 2), Dry Ice (Box 1) |
| Storage Conditions | Room Temperature and –15 to –25 °C |
Kit Contents & Storage
Box 1 (Store at –15 to –25 °C):
- Proteinase K, 20 mg/mL – 0.48 mL
- Lysis/Binding Enhancer – 0.96 mL
- TURBO™ DNase, 20 U/µL – 0.2 mL
Box 2 (Store at Room Temperature):
- Lysis Buffer – 115 mL
- PK Digestion Buffer – 4.4 mL
- RNA Binding Beads – 2 mL
- Wash Solution 1 Concentrate – 20 mL
- Wash Solution 2 Concentrate – 60 mL
- Rebinding Buffer – 4.8 mL
- TURBO DNase Buffer – 4.8 mL
- Elution Buffer – 9.6 mL
- 1 Processing Plate, 2 Elution Plates, 4 Plate Covers
Comparison with Related RNA Isolation Kits
| Kit Name | RNA Targets | Sample Types | Throughput Format | Key Features |
|---|---|---|---|---|
| MagMAX™ mirVana™ Total RNA Kit | Total RNA + small RNA | Serum, plasma, tissue, urine, cells | 6–96 samples (manual/automated) | Small RNA recovery; phenol-free; miRNA-compatible workflows |
| MagMAX™ Total RNA Isolation Kit | Total RNA | Cell culture, tissue, blood | High-throughput (KingFisher) | High-yield total RNA, no DNase included |
| mirVana™ miRNA Isolation Kit | miRNA, small RNA | Tissue, cells, plasma | Manual | Phenol-chloroform method for maximum miRNA recovery |
| PureLink™ RNA Mini Kit | Total RNA | Cells, tissues | Manual | Column-based RNA isolation |
Unique Advantages of the MagMAX™ mirVana™ Total RNA Kit
- Comprehensive RNA Isolation: Captures both large and small RNAs for full transcriptome profiling.
- Flexible Sample Handling: One kit supports a wide range of biological samples—no need for specialized alternatives.
- Automation Ready: Compatible with leading KingFisher™ platforms, from research-scale to high-throughput.
- No Phenol, No Columns: Magnetic bead purification ensures cleaner, safer, and clog-free workflows.
- Downstream Application-Ready: Delivers high-quality RNA for sensitive assays like miRNA-Seq, RT-qPCR, and more.