Liofilchem® Teicoplanin TEC Antimicrobial Susceptibility Discs, 30 µg

The Liofilchem® Teicoplanin TEC Antimicrobial Susceptibility Discs (30 µg) are standardized discs used for determining the antimicrobial susceptibility of bacteria to Teicoplanin. Teicoplanin is a glycopeptide antibiotic effective against a variety of gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA) and Enterococcus spp. These discs are essential for clinical microbiology laboratories conducting antibiotic susceptibility testing (AST) to guide therapeutic decisions.

Key Features:

1. Active Agent:
   • Teicoplanin (30 µg): A glycopeptide antibiotic that inhibits bacterial cell wall synthesis by binding to the D-Ala-D-Ala terminus of peptidoglycan precursors.
2. Standardization:
   • Conforms to EUCAST and CLSI guidelines for antimicrobial susceptibility testing.
3. Applications:
   • Used in Kirby-Bauer disc diffusion tests to determine bacterial susceptibility or resistance to Teicoplanin.
4. Packaging and Quality:
   • Individually packaged to ensure sterility and stability.
   • Manufactured with consistent and precise antibiotic concentration.

Specifications:

Use Cases:

1. Clinical Microbiology:
   • Identify antibiotic susceptibility patterns of gram-positive pathogens such as MRSA, Staphylococcus epidermidis, and Enterococcus faecium.
   • Monitor the emergence of resistance to Teicoplanin in healthcare-associated infections.
2. Pharmaceutical Research:
   • Evaluate the efficacy of Teicoplanin against clinical isolates in drug development and pharmacological studies.
3. Antibiotic Stewardship Programs:
   • Support programs aimed at optimizing antibiotic use by accurately detecting resistant bacterial strains.
4. Hospital and Diagnostic Laboratories:
   • Standardized testing in routine diagnostics to guide appropriate antibiotic therapy.

Testing Protocol (Kirby-Bauer Disc Diffusion Method):

1. Preparation:
   • Use Mueller-Hinton agar as the standard medium for testing.
   • Inoculate the agar plate with a standardized bacterial suspension (0.5 McFarland standard).
2. Application:
   • Place the Teicoplanin 30 µg disc on the inoculated agar surface using sterile forceps.
3. Incubation:
   • Incubate the plate at 35°C ± 2°C for 16-18 hours under aerobic conditions.
4. Measurement:
   • Measure the diameter of the zone of inhibition and interpret results according to EUCAST or CLSI guidelines.

Advantages:

• Accuracy: Provides precise and reproducible results for antimicrobial susceptibility testing.
• Guidelines Compliance: Adheres to globally recognized standards for AST.
• Versatility: Effective for a wide range of gram-positive bacterial isolates.
• Convenience: Ready-to-use format simplifies laboratory workflows.

References:

1. EUCAST Guidelines: European Committee on Antimicrobial Susceptibility Testing.
   https://www.eucast.org
2. CLSI Guidelines: Clinical and Laboratory Standards Institute.
   https://clsi.org
3. O’Driscoll, T., et al. "Methicillin-resistant Staphylococcus aureus (MRSA): epidemiology, clinical management, and evolution." Clinical Microbiology Reviews, 2010.
4. Hiramatsu, K., et al. "The emergence and evolution of methicillin-resistant Staphylococcus aureus." Trends in Microbiology, 2001.

The Liofilchem® Teicoplanin TEC Antimicrobial Susceptibility Discs (30 µg) are indispensable tools for assessing the efficacy of Teicoplanin against gram-positive bacterial pathogens. By enabling standardized and reliable susceptibility testing, these discs support clinical decision-making, research efforts, and antibiotic stewardship initiatives in combating resistant bacterial infections.