Liofilchem® Gentamicin CN, Antimicrobial Susceptibility Discs, 10 µg (copy)

Liofilchem® Amikacin Antimicrobial Susceptibility Discs, 30 µg, are precision-manufactured antibiotic discs used for determining the susceptibility of microorganisms to amikacin. These discs are used in standardized procedures such as the Kirby-Bauer disk diffusion method for antimicrobial susceptibility testing. The 30 µg amikacin discs are impregnated with a defined quantity of the aminoglycoside antibiotic amikacin, making them ideal for identifying bacterial resistance patterns in clinical and research settings.

Amikacin is a potent broad-spectrum aminoglycoside antibiotic effective against a wide range of Gram-negative and some Gram-positive bacteria. It is commonly used to treat infections caused by multidrug-resistant organisms, particularly in nosocomial settings.

Features:

1. Standardized Potency: Each disc contains 30 µg of amikacin, conforming to international standards such as CLSI and EUCAST.
2. Precise Diameter: Manufactured to ensure uniform size and antibiotic diffusion for reliable and reproducible results.
3. Convenient Packaging: Supplied in cartridges or blister packs, protected from moisture and contamination.
4. Quality Assurance: Undergo rigorous quality control testing for potency, diffusion consistency, and sterility.
5. Compatibility: Suitable for use with standard agar diffusion methods like Kirby-Bauer and compatible with various susceptibility testing systems.

Composition:

• Active Ingredient: 30 µg amikacin per disc.
• Carrier: High-quality filter paper for uniform diffusion.
• Packaging Material: Moisture-resistant containers with desiccants to ensure product integrity.

Method of Use:

1. Preparation:
   • Prepare Mueller-Hinton Agar plates according to standardized protocols.
   • Allow the medium to solidify and reach room temperature.
2. Inoculation:
   • Prepare a bacterial suspension equivalent to a 0.5 McFarland standard.
   • Evenly inoculate the agar surface using a sterile swab to create a lawn of growth.
3. Application of Discs:
   • Using a sterile disc dispenser or forceps, place the Amikacin 30 µg discs onto the agar surface.
   • Ensure the discs are evenly spaced and at least 24 mm apart from center to center.
4. Incubation:
   • Invert the plates and incubate aerobically at 35 ± 2°C for 16-18 hours.
5. Interpretation:
   • Measure the diameter of the zone of inhibition around the disc in millimeters.
   • Compare the measurements to interpretive criteria provided by CLSI/EUCAST to classify the isolate as susceptible, intermediate, or resistant.

Quality Control:

Recommended control strains to verify performance:

• Escherichia coli ATCC® 25922: Expected zone range: 19-25 mm.
• Pseudomonas aeruginosa ATCC® 27853: Expected zone range: 19-25 mm.
• Staphylococcus aureus ATCC® 25923: Expected zone range: 19-25 mm.

Storage:

• Store at 2-8°C in the original container with desiccant.
• Protect from moisture, light, and temperature fluctuations.
• Do not use beyond the expiry date indicated on the packaging.

Applications:

1. Clinical Microbiology Laboratories:
   • Detection of antimicrobial resistance in Gram-negative and some Gram-positive bacteria.
2. Pharmaceutical Research:
   • Evaluation of antibiotic efficacy against clinical isolates.
3. Public Health Monitoring:
   • Surveillance of antibiotic resistance trends.

Limitations:

• Results should be interpreted in conjunction with other clinical and microbiological data.
• Not suitable for direct use on non-standardized or non-validated media.
• Disc performance may be affected by improper storage conditions.

Regulatory Compliance:

• Manufactured in accordance with CLSI/EUCAST guidelines.
• CE-marked for in vitro diagnostic use.

Safety Precautions:

• For in vitro diagnostic use only.
• Use by trained laboratory professionals.
• Dispose of used materials according to institutional biosafety protocols.

References:

1. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing.
2. European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines.