Liofilchem® Pipemidic acid PI, Antimicrobial Susceptibility Discs, 20 µg

Liofilchem® Pipemidic Acid (PI) Antimicrobial Susceptibility Discs (20 µg) are precision-formulated paper discs designed for in vitro determination of bacterial susceptibility to pipemidic acid. Each disc contains 20 micrograms of the active compound, a first-generation quinolone antibiotic primarily used in urinary tract infection (UTI) pathogen profiling. The discs are optimized for use in Kirby-Bauer disk diffusion tests to evaluate the activity of pipemidic acid against Gram-negative uropathogens, including Escherichia coli, Proteus spp., Klebsiella spp., and Enterobacter spp.

Manufactured under ISO 9001 and ISO 13485 standards, these discs deliver consistent and reproducible results for use in clinical, research, and veterinary microbiology laboratories.

Key Features

• Standardized potency: Each disc is loaded with 20 µg of pipemidic acid to ensure accurate and reproducible inhibition zones.
• Targeted antimicrobial activity: Effective primarily against Gram-negative bacilli, especially those involved in urinary tract infections.
• Diagnostic value: Used to assess susceptibility in Enterobacteriaceae, including E. coli, Proteus, Klebsiella, and Citrobacter species.
• CLSI/EUCAST-aligned: Can be interpreted in conjunction with recognized zone diameter guidelines or laboratory-specific breakpoints.
• Sterile and stable: Supplied in sealed cartridges or canisters with desiccant to protect disc integrity during transport and storage.
• Color-coded labeling: Assists rapid identification in multi-disc testing workflows.

Applications and Use Cases

1. Clinical Microbiology

• Empirical and confirmatory testing of urinary tract pathogens for pipemidic acid susceptibility.
• Alternative quinolone screening when other fluoroquinolones are contraindicated or unavailable.

2. Veterinary Microbiology

• Susceptibility profiling of Gram-negative isolates in animal UTIs, particularly in small animal practice.

3. Pharmaceutical and Research Laboratories

• Evaluation of pipemidic acid activity against resistant or emerging uropathogens.
• Comparative analysis with fluoroquinolones like nalidixic acid, norfloxacin, or ciprofloxacin.

Use Instructions

1. Prepare the medium: Mueller-Hinton Agar (MHA) is recommended.
2. Inoculum standardization: Adjust the test organism to 0.5 McFarland turbidity.
3. Inoculate plate: Swab evenly across the agar surface with a sterile cotton swab.
4. Apply disc: Place the 20 µg Pipemidic acid disc using sterile forceps or a disc dispenser.
5. Incubate:
   • Temperature: 35 ± 2 °C
   • Duration: 16–18 hours in ambient air
6. Interpretation:
   • Measure the zone of inhibition (mm) around the disc.
   • Compare to internal lab guidelines or established references (CLSI/EUCAST, if applicable).

Technical Specifications

Quality Control

Quality assurance is performed using standard bacterial reference strains:

Liofilchem® Pipemidic Acid PI Discs (20 µg) provide an effective tool for the detection of quinolone susceptibility in uropathogens. Ideal for clinical and veterinary diagnostic labs, these discs contribute to evidence-based antibiotic therapy selection in urinary tract infections. With ISO-certified manufacturing, precise potency, and broad application utility, they support reliable and consistent antimicrobial resistance profiling.