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Thermo Scientific™ NAb™ Protein L Spin Kit, 0.2 mL, 10 columns kit
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Specifications:
| Application | Protein Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | columns, Molecular Biology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ NAb™ Protein L Spin Kit, 0.2 mL is a robust and efficient system designed for the rapid, small-scale affinity purification of antibodies that contain kappa light chains. This kit is particularly beneficial when working with monoclonal antibodies or antibody fragments that do not bind effectively to Protein A or G, such as IgM, IgA, or scFv/Fab fragments.
Key Features
- Target-Specific Binding: Contains immobilized recombinant Protein L, which selectively binds to antibodies with kappa light chains through their variable light domains (VLκ) rather than the Fc region.
- High Purity and Yield: Each 0.2 mL column enables purification of 100–1,000 μg of IgG from 25–500 μL of complex biological samples such as serum or hybridoma supernatants.
- Pre-packed Convenience: Supplied in ready-to-use spin column format, each kit includes 10 columns, collection tubes, and required buffers, streamlining your purification workflow.
- Broad Immunoglobulin Class Compatibility: Suitable for antibodies across multiple classes—IgG, IgA, IgM, IgE, and IgD—as long as they feature compatible kappa light chains.
Applications
- Purification of monoclonal or polyclonal antibodies that have poor affinity for Protein A/G.
- Selective enrichment of scFv and Fab antibody fragments from expression supernatants.
- Immunoprecipitation and co-immunoprecipitation assays, especially with IgM antibodies or when preserving antigen-binding activity is essential.
- Antibody recovery from serum-supplemented culture media, where Protein L does not bind bovine serum immunoglobulins, reducing contamination.
Technical Specifications
| Property | Details |
|---|---|
| Column Format | Microcentrifuge Spin Column (0.2 mL resin bed) |
| Binding Capacity | 5–10 mg IgG/mL resin |
| Purification Range per Column | 100–1,000 μg IgG |
| Resin Type | Crosslinked 6% beaded agarose (CL-6B) |
| Ligand | Recombinant Protein L |
| Target Binding Site | Variable light chains (kappa only) |
| Compatible Light Chain Subtypes | Human VkI, VkIII, VkIV; Mouse VkI |
| Incompatible | Lambda light chains, human VkII |
| pH Stability | 2–14 (short-term), 3–13 (long-term) |
| Average Particle Size | 45–165 μm |
| Maximum Flow Rate | ~1 mL/min (1 cm diameter column) |
| Storage Temperature | 4°C |
| Shipping Condition | Ambient |
The NAb™ Protein L Spin Kit offers a rapid, reliable solution for the purification of antibodies and antibody fragments that fall outside the conventional Protein A/G range. With high specificity for kappa light chains and minimal non-specific binding, it is ideal for sensitive and low-volume purifications in research and development applications.