Liofilchem LCAT supplement

The Liofilchem™ L.C.A.T. Supplement is a selective, freeze-dried additive specifically designed for the preparation of G.C. Medium (Codes 610022 and 620022) to facilitate the cultivation of Neisseria spp. This supplement contains a combination of antibiotics and antifungal agents that selectively promote the growth of Neisseria spp. while inhibiting unwanted bacteria, fungi, and yeasts.

Key Features

1. Selective Growth Promotion:
   • Supports the growth of Neisseria spp. by inhibiting competing Gram-positive bacteria, Gram-negative bacteria, fungi, and yeasts.
2. Pre-Measured Convenience:
   • Each vial is pre-measured for 500 mL of G.C. Medium, ensuring consistency and ease of preparation.
3. Validated for Clinical Applications:
   • Tested with control strains such as Neisseria gonorrhoeae and Neisseria meningitidis to ensure reliability.
4. Freeze-Dried Stability:
   • Long shelf life and easy storage at 2–8°C.

Kit Contents

• 10 vials of freeze-dried L.C.A.T. Supplement.
• 1 instruction sheet.

Composition

Applications

1. Clinical Microbiology:
   • Cultivation and isolation of Neisseria gonorrhoeae and Neisseria meningitidis from clinical specimens.
2. Food and Pharmaceutical Testing:
   • Monitoring contamination by Neisseria spp. in specific microbiological workflows.
3. Research:
   • Studies focusing on Neisseria species and their growth dynamics.

Preparation Instructions

1. Reconstitute the Supplement:
   • Aseptically reconstitute the contents of one vial with 10 mL of sterile distilled water.
   • Mix until fully dissolved.
2. Add to G.C. Medium:
   • Add the reconstituted solution to 500 mL of G.C. Medium (autoclaved and cooled to 45-50°C).
   • Enrich the medium with:
     • 5% defibrinated horse blood (cooked to chocolate agar).
     • 1 vial of Vitalex growth supplement reconstituted with 5 mL of Vitalex restoring solution (Code 81023).
3. Mix Thoroughly:
   • Distribute into sterile Petri dishes.

Quality Control

• Appearance:
  • Freeze-dried powder, pale yellow in color.
• Microbiological Control:
  • Inoculate plates prepared with G.C. Medium supplemented with L.C.A.T. Supplement.
  • Incubate at 36 ± 1°C for 48–72 hours in a 5–10% CO₂ atmosphere.
• Control Strains:

  Control Strain	Growth
  Neisseria gonorrhoeae	Good
  Neisseria meningitidis	Good
  Escherichia coli	Inhibited
  Staphylococcus aureus	Inhibited

Storage and Shelf Life

• Storage:
  • Store at 2–8°C in original packaging.
  • Once reconstituted, store at -20°C for up to 30 days, protected from light.
• Shelf Life:
  • Valid until the expiry date indicated on the label.

Advantages

• Highly Selective:
  • Facilitates the isolation of Neisseria spp. while suppressing unwanted microbial growth.
• Validated Performance:
  • Tested with clinical strains to ensure reliable results.
• Convenient:
  • Pre-measured vials for consistent medium preparation.
• Long Shelf Life:
  • Freeze-dried format ensures stability and ease of storage.

Warnings and Precautions

1. Contains substances classified as hazardous—handle with care and consult the Safety Data Sheet (SDS).
2. For in vitro diagnostic use (IVD) only.
3. Use only in professional laboratory settings with trained personnel.
4. Dispose of unused or expired product according to local regulations.

Packaging

References

1. Chapin C.K., G.V. Doern (1983). J. Clin. Microbiol. 17:1163–1165.
2. Martin J.E., Armstrong J.H., Smith P.B. (1974). Appl. Microbiol. 27:802–805.
3. NCCLS M22-A2 (1996). Quality Assurance for Commercially Prepared Microbiological Culture Media.

Why Choose Liofilchem L.C.A.T. Supplement?

• Reliable Selectivity: Ensures suppression of non-target organisms for accurate Neisseria spp. cultivation.
• Ease of Use: Pre-measured vials simplify preparation and reduce errors.
• Validated: QC-tested for optimal performance in clinical microbiology.