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Liofilchem® Methylene Blue Solution 0.3%, 250 mL
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Specifications:
| Application | Clinical microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Biological Stain | Forms | Liquid |
| Product Brand | Liofilchem | ||
| Product Grade | Microbiology grade | ||
Ready-to-Use Biological Stain for Microbiology, Hematology, and Cell Viability Applications
The Liofilchem® Methylene Blue Solution 0.3% is a ready-to-use aqueous staining solution widely used in microbiology, hematology, and cell biology for the visualization and differentiation of microorganisms, blood cells, and cellular structures. Supplied in a 250 mL laboratory-grade bottle, this solution contains 0.3% methylene blue in water, formulated for consistent staining results and broad laboratory use.
Methylene blue is a basic dye that binds to acidic cellular components, particularly nucleic acids, making it useful for staining nuclei, bacteria, and certain parasites. It is commonly applied in Gram staining procedures, simple bacterial morphology assessments, and viability assays such as yeast and cell culture evaluations.
✅ Key Features & Benefits
- Concentration: 0.3% methylene blue in aqueous solution
- Ready-to-Use: No dilution or preparation required
- High-Quality Staining: Suitable for microscopy and microbiological assays
- Multipurpose Use: Ideal for bacterial cell staining, yeast viability testing, wet mount preparations, and RBC differentiation
- Laboratory Grade: Manufactured under quality-controlled conditions for consistent results
- Convenient Packaging: Supplied in a 250 mL reagent bottle for extended lab use
- Shelf-Stable: Long shelf life when stored under appropriate conditions
🧪 Applications
- Microbiology: Basic staining for bacterial morphology and simple identification
- Hematology: Nuclear staining and differential analysis of blood smears
- Cell Culture: Cell viability assays and exclusion testing (e.g., yeast viability)
- Parasitology: Highlighting protozoan and helminth structures in stool samples
- Wet Mount Microscopy: Live-cell visualization and contrast enhancement
⚙ Product Specifications
| Attribute | Details |
|---|---|
| Product Name | Methylene Blue Solution 0.3% |
| Brand | Liofilchem® |
| Concentration | 0.3% methylene blue |
| Volume | 250 mL |
| Formulation | Aqueous solution |
| Packaging | Plastic laboratory bottle |
| Storage Conditions | Room temperature, away from light |
| Intended Use | In vitro laboratory use only |
🔬 Instructions for Use (General Guidelines)
- Place a drop of Methylene Blue 0.3% solution on the sample slide.
- Apply the specimen (e.g., bacterial smear, yeast culture, or blood smear).
- Allow to stain for 30 seconds to 2 minutes, depending on sample and purpose.
- Rinse gently with distilled water and examine under a microscope.
⚠ For viability staining, mix methylene blue directly with the sample before visualization.
⚠ Safety & Handling
- For in vitro use only – Not for therapeutic or diagnostic use
- Use gloves and eye protection during handling
- Avoid ingestion or contact with mucous membranes
- Dispose of according to local chemical waste regulations
- Refer to Material Safety Data Sheet (MSDS) for detailed safety information
The Liofilchem® Methylene Blue Solution 0.3%, 250 mL is a versatile and essential staining reagent in microbiological and biomedical laboratories. With its reliable performance, easy application, and broad utility, this solution supports a wide range of routine staining protocols and viability assays, making it a staple in laboratory diagnostics, academic teaching, and research environments.