Liofilchem™ Enterosystem 18R Reagents for 100 Tests

Reagents for Indole and Voges-Proskauer (VP) tests, as well as anaerobic maintenance in select wells of the Enterosystem 18R, designed for the identification of Gram-negative, oxidase-negative Enterobacteria.

Description

The Liofilchem™ Enterosystem 18R Reagent kit contains the essential chemical reagents to perform:

• The Voges-Proskauer (VP) test in well 10-VP.
• The Indole test in well 11-IND.
• Anaerobic maintenance in wells 2-LDC, 3-ODC, 4-ADC, 7-UR, and 8-H2S using Vaseline Oil.

This kit ensures accurate biochemical reactions and reliable results for the Enterosystem 18R bacterial identification panel.

Kit Contents

Principle of the Method

• Voges-Proskauer Test:
  • Detects the production of acetoin from glucose fermentation.
  • Reagents Used: Alpha Naphtol and NaOH 40%.
  • Positive Result: Pink to red color in well 10-VP.
• Indole Test:
  • Identifies the ability of bacteria to produce indole from tryptophan degradation.
  • Reagent Used: Kovac's Reagent.
  • Positive Result: Formation of a red/pink layer in well 11-IND.
• Anaerobic Maintenance:
  • Vaseline Oil ensures an oxygen-restricted environment for specific biochemical reactions (e.g., lysine, ornithine, arginine decarboxylation).

Reagents

Test Procedure

1. Voges-Proskauer Test (Well 10-VP):
   • Add Alpha Naphtol followed by NaOH 40% to the well.
   • Mix thoroughly and allow 10–20 minutes for color development.
   • Positive Result: Pink/red color.
2. Indole Test (Well 11-IND):
   • Add Kovac's Reagent to the well.
   • Observe for a red/pink layer within a few minutes.
   • Positive Result: Red/pink color.
3. Anaerobic Maintenance (Wells 2-LDC, 3-ODC, 4-ADC, 7-UR, 8-H2S):
   • Seal the wells with Vaseline Oil to create an anaerobic environment.

Refer to the Enterosystem 18R Package Insert for detailed protocols.

Quality Control

• Validate reagent performance using suitable control organisms:
  • Positive Controls:
    • Escherichia coli: Positive for indole; negative for Voges-Proskauer.
    • Enterobacter cloacae: Positive for Voges-Proskauer; negative for indole.
  • Negative Controls:
    • Strains with no indole or acetoin production.

Storage and Stability

• Store reagents at 2–8°C in their original packaging, protected from light.
• Stability:
  • Can tolerate short-term storage or transport at 18–25°C for 4 days or 35–39°C for 48 hours without performance loss.
• Discard reagents if signs of contamination or deterioration are observed.

Precautions

• For in vitro diagnostic use only.
• Classified as hazardous:
  • Follow safety protocols as outlined in the Safety Data Sheet (SDS).
• Use by trained personnel with appropriate aseptic techniques.
• Dispose of used reagents and contaminated materials according to laboratory guidelines for biohazard waste.

Applications

• Clinical Diagnostics:
  • Identification of Enterobacteriaceae in infections like sepsis, UTIs, and gastroenteritis.
• Industrial Microbiology:
  • Quality control for food, water, and pharmaceutical products.
• Research and Education:
  • Biochemical profiling of bacterial species.

Presentation

Bibliography

1. Murray, Baron, Pfaller, Tenorev, Yolken. Manual of Clinical Microbiology (1995).
2. Bayley and Scott. Diagnostic Microbiology (1986).
3. Edwin H. Lenette. Manual of Clinical Microbiology (1995).

The Liofilchem™ Enterosystem 18R Reagents are critical components for performing biochemical tests with the Enterosystem 18R, ensuring precise and reliable identification of Gram-negative, oxidase-negative Enterobacteria.