BioTek 800TS Microplate Absorbance reader with GE5 Software

• High quality, high-performance microplate reading at an affordable price. Robust instrument hardware and powerful software, along with unparalleled service and support, make Agilent BioTek the choice for high-performance plate readers
• The BioTek 800 TS enables a variety of workflows including ELISA, protein, and other endpoint protocols. Incubation and shaking expand the reach to enzyme kinetics and cell-based assays, providing automated operation in your laboratory
• Color touch screen for quick, easy programming and operation with menu-driven software for protocol creation. Intuitive and simple to use, the results are delivered quickly and reliably while protocols and data are saved onboard for instant recall
• Very precise and accurate results at an affordable price. High-quality hardware and optical design ensure excellent performance for all assays. Easily verify and qualify performance using the absorbance test plate and product qualification package
• Flexible and convenient data utilization with both USB flash drive for data export and transportability and Gen6 software import for analysis. Gen6 software offers advanced data handling and custom reporting.

Specifications

How It Works

Ease of use

The Agilent BioTek 800 TS absorbance reader offers a simple reading, data visualization, results reporting, and data export procedure.

Agilent BioTek Gen6 compatibility

The Agilent BioTek 800 TS absorbance reader can be integrated with the new Agilent BioTek Gen6 software to easily perform advanced data analysis. For laboratories that need to meet the requirements of 21 CFR Part 11, Agilent BioTek Gen5 Secure is available.

6- to 384-well plate compatibility

The Agilent BioTek 800 TS absorbance reader can read assays in 6- to 384-well formats, providing the versatility to suit a wide range of applications.

Applications

Enzyme kinetics and cell-based assays

The 800 TS offers temperature control to 50 °C, and shaking. Under Gen6 control, kinetic measurements and analysis are enabled.

Pair with the Agilent BioTek 50 TS washer for semi-automated ELISA

An 800 TS absorbance reader, partnered with the Agilent BioTek 50 TS washer, creates a compact, yet efficient semi-automated system for ELISA processes.

Technology

Absorbance refers to the ability of a substance to absorb light of a specific wavelength. In a microplate reader designed to measure absorbance, a light source illuminates the sample using a specific wavelength, selected by an optical filter or a monochromator. A light detector located on the other side of the well measures how much of the initial light is transmitted through the sample. The amount of transmitted light will typically be related to the concentration of the molecule of interest, and the result is referred to as the optical density (OD) or absorbance of the sample. Absorbance is calculated as shown in the equation:

A λ = Log 10 (I 0 /I)

Where I is the intensity of light at a specified wavelength λ that has passed through a sample (transmitted light intensity) and I 0/I is the intensity of the light before it enters the sample.

Typical Colorimetric Assay

In a typical colorimetric assay, an enzyme/substrate reaction causes a color change in the samples in the microplate.

Optical Density (OD)

Light at a specific wavelength is passed through the sample to a detector. The amount of light absorbed by the sample is referred to as the optical density (OD) or absorbance.

Many conventional colorimetric analyses have been or can be miniaturized for measurement in a microplate reader. The Agilent BioTek microplate readers and Agilent BioTek Gen6 software can facilitate assay miniaturization to microplates by providing optional cuvette ports and automated pathlength correction. Absorbance detection in microplate readers is used for assays such as ELISA assays, protein and nucleic acid quantification and purity assays, endotoxin analysis, and many others.