Liofilchem®Strepto System 12R, 40 Tests

The Liofilchem® Streptosystem 12R is a comprehensive system for the identification of streptococci based on biochemical tests. This 24-well system evaluates 12 biochemical reactions across two tests per system, making it a valuable tool for clinical diagnostics, microbiology research, and laboratory workflows.

Key Features:

1. Comprehensive Identification:
   • Differentiates a wide range of Streptococcus spp. and related organisms.
   • Utilizes 12 desiccated biochemical substrates for accurate identification.
2. Numerical Code System:
   • Reactions produce a 4-digit numerical code for easy identification using a codes table or software.
3. Ready-to-Use:
   • Pre-filled wells with desiccated substrates reduce preparation time and ensure consistency.
4. Two Tests per System:
   • Each system includes 24 wells, allowing the performance of two separate tests per setup.
5. Rapid Workflow:
   • Results available within 18–24 hours after incubation.

Applications:

1. Clinical Microbiology:
   • Identifies pathogenic streptococci from clinical specimens to aid in diagnostics.
2. Research Microbiology:
   • Supports studies in microbial differentiation and metabolic pathways.
3. Industrial Microbiology:
   • Ensures quality control in pharmaceutical and food microbiology.

Kit Contents:

Biochemical Reactions:

Procedure:

1. Preparation of Bacterial Suspension:
   • Isolate the microorganism from an agar plate and suspend in Strepto Broth.
2. Inoculate System:
   • Transfer 0.2 mL of bacterial suspension into each well.
   • Add PYR disc to well 4-PYR.
   • Overlay well 6-ADC with vaseline oil.
3. Incubation:
   • Cover the system and incubate at 36 ± 1°C for 18–24 hours.
4. Interpret Results:
   • Add reagents to specific wells as needed (e.g., VP, IPP, and PYR tests).
   • Record color changes and calculate the 4-digit numerical code using the provided instructions.

Numerical Code Formation:

• Tests are grouped into four groups of three:
  • Group 1: VP, IPP, ESC
  • Group 2: PYR, ONPG, ADC
  • Group 3: RIB, ARA, MAN
  • Group 4: SOR, TRE, RAF
• Assign values (1, 2, or 4) based on positive tests and sum values in each group to form a 4-digit code.
• Use the code table or online software to identify the microorganism.

Quality Control:

• Control Strains:
  • Streptococcus pyogenes ATCC® 19615
  • Streptococcus pneumoniae ATCC® 6303
  • Enterococcus faecalis ATCC® 19433
  • Streptococcus agalactiae ATCC® 13813
• Expected Reactions:
  • Positive and negative control strains ensure test accuracy.

Storage and Handling:

• Store at 2–8°C in the original packaging.
• Avoid excessive temperature fluctuations.
• Discard systems if deterioration or contamination is evident.

Specifications:

Advantages:

1. Comprehensive and Accurate:
   • Identifies a wide range of streptococcal species with clinical relevance.
2. Time-Efficient:
   • Provides results in under 24 hours, expediting diagnostics.
3. Easy to Use:
   • Pre-filled wells and clear instructions simplify the workflow.
4. Validated Performance:
   • Rigorous quality control ensures consistent and reliable results.

Product Details:

The Liofilchem® Streptosystem 12R is an indispensable tool for clinical and research microbiology laboratories, offering comprehensive identification of streptococcal species with precision and efficiency. Its pre-filled, ready-to-use design and rapid workflow make it a trusted choice for bacterial identification.