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Specifications:
| Application | Clinical microbiology |
| Storage Temperature | 2-8°C |
| Product Type | Microbial ID System |
| Product Brand | Liofilchem |
| Product Grade | Microbiology grade |
Biochemical Identification of Gram-negative, Oxidase-negative Enterobacteria
The Liofilchem® Enterosystem 18R is a diagnostic system designed to accurately identify Gram-negative, oxidase-negative bacteria, particularly Enterobacteria, through 18 biochemical reactions. This system simplifies microbial identification with easy-to-read color changes, rapid results, and compatibility with automatic reading systems.
Key Features
- Comprehensive Identification:
- Detects and identifies a broad range of Enterobacteria, including Escherichia coli, Shigella spp., Salmonella spp., Klebsiella spp., Enterobacter spp., Serratia spp., Proteus spp., and Yersinia spp.
- Efficient Workflow:
- Incubation Time: Results available in 12, 18, or 24 hours depending on the microorganism and growth conditions.
- Clear, well-defined color changes for straightforward result interpretation.
- Ease of Use:
- Compact system with all necessary biochemical substrates in one panel.
- Compatible with automatic reading systems for high-throughput laboratories.
- Cost-Effective:
- Provides complete identification in a single test, reducing the need for additional diagnostic procedures.
Biochemical Reactions
The system evaluates 18 biochemical reactions, targeting essential metabolic activities such as:
- Carbohydrate fermentation
- Decarboxylation of amino acids
- Hydrolysis of urea
- Production of hydrogen sulfide
- Detection of enzymatic activities
Microorganisms Identified
| Genus | Species Identified |
|---|---|
| Escherichia | E. coli |
| Shigella | S. dysenteriae, S. flexneri, S. boydii, S. sonnei |
| Citrobacter | C. freundii, C. diversus, C. amalonaticus |
| Salmonella | S. typhi, S. cholerae suis, S. paratyphi A, S. arizonae, S. pullorum |
| Klebsiella | K. pneumoniae, K. oxytoca, K. ozaenae, K. rhinoscleromatis |
| Enterobacter | E. aerogenes, E. cloacae, E. agglomerans, E. sakazakii, E. alvei |
| Serratia | S. liquefaciens, S. marcescens, S. rubidaea, S. odorifera, S. plymuthica |
| Proteus | P. vulgaris, P. mirabilis, P. penneri |
| Morganella | M. morganii |
| Providencia | P. rettgeri, P. stuartii, P. alcalifaciens, P. rustigiani |
| Yersinia | Y. enterocolitica, Y. pestis, Y. intermedia, Y. pseudotuberculosis |
Test Procedure
- Inoculation:
- Inoculate the Enterosystem 18R panel with a bacterial suspension prepared according to the protocol.
- Incubation:
- Incubate at 36 ± 1°C for 12, 18, or 24 hours depending on the microorganism.
- Result Interpretation:
- Observe the color changes in the wells.
- Record positive and negative reactions to generate a biochemical profile code.
- Identification:
- Use the online ID Software or the Enterosystem Code Book (ref. 71710) to match the biochemical code to the corresponding bacterial species.
Benefits
| Feature | Benefit |
|---|---|
| Complete Identification | Identifies a wide range of clinically significant Enterobacteria. |
| Rapid Results | Delivers results in as little as 12 hours, aiding timely clinical decisions. |
| Cost-Effective | Combines all necessary tests in one compact system, reducing costs. |
| Clear Color Changes | Ensures easy and accurate interpretation of results. |
| Automation Compatible | Supports automatic reading for high-throughput settings. |
Applications
- Clinical Microbiology:
- Identifies Enterobacteria in infections such as urinary tract infections, gastrointestinal diseases, and sepsis.
- Industrial Microbiology:
- Monitors contamination in food, water, and pharmaceutical products.
- Research:
- Supports studies on bacterial metabolism and resistance mechanisms.
Packaging Information
| Product | Description | Packaging | Reference |
|---|---|---|---|
| Enterosystem 18R | Biochemical ID panel for Gram-negative bacteria | 20 tests | 71618 |
| Enterosystem Reagent | Reagent for specific reactions | 100 tests | 80252 |
| Code Book | Identification guide for Enterobacteria | 1 book | 71710 |
Why Choose Liofilchem® Enterosystem 18R?
- Comprehensive Coverage: Identifies over 30 species of clinically relevant Enterobacteria.
- Time-Saving: Provides results within 12–24 hours, expediting diagnostic workflows.
- User-Friendly: Simple inoculation and interpretation process with clear colorimetric results.
- Flexible Options: Compatible with both manual and automated result interpretation.
The Liofilchem® Enterosystem 18R and the API 20E are both diagnostic tools designed for the identification of Gram-negative, oxidase-negative Enterobacteriaceae. Below is a detailed comparison of their features:
| Feature | Liofilchem® Enterosystem 18R | API 20E |
|---|---|---|
| Manufacturer | Liofilchem S.r.l. | bioMérieux |
| Test Principle | Utilizes 18 biochemical substrates to assess metabolic activities, leading to the identification of Enterobacteriaceae species. | Employs 21 standardized biochemical tests to evaluate enzymatic activities for bacterial identification. |
| Incubation Time | Results available in 12 to 24 hours, depending on the microorganism and growth conditions. | Typically requires 18 to 24 hours of incubation at 36°C for result interpretation. |
| Result Interpretation | Observes color changes in wells; generates a numerical code matched against a codebook or software for species identification. | Assesses color changes in microtubes; produces a profile number compared to a database for species identification. |
| Automation Compatibility | Compatible with automatic reading systems, facilitating high-throughput analysis. | Can be read manually or with automated systems like the ATB Expression reader. |
| Scope of Identification | Identifies a wide range of Enterobacteriaceae, including Escherichia coli, Shigella spp., Salmonella spp., Klebsiella spp., Enterobacter spp., Serratia spp., Proteus spp., and Yersinia spp. | Covers a broad spectrum of Enterobacteriaceae and other Gram-negative bacteria |
| Cost-Effectiveness | Offers a cost-effective solution with all necessary reagents included in a single panel. | Generally more expensive; may require additional reagents or equipment for certain tests. |
| Ease of Use | Features clear and well-defined color changes for straightforward interpretation; includes a codebook for result analysis. | Provides detailed instructions and a comprehensive database for result interpretation; may require more training for accurate use. |
| Additional Features | Includes reagents for specific tests, such as indole and Voges-Proskauer, enhancing the identification process. | Offers a wide array of tests, including those for less common metabolic activities, providing a comprehensive identification profile. |
In summary, both the Liofilchem® Enterosystem 18R and the API 20E are effective systems for identifying Enterobacteriaceae. The Enterosystem 18R is noted for its rapid results, cost-effectiveness, and user-friendly design, making it suitable for laboratories seeking efficient and straightforward bacterial identification. The API 20E offers a more extensive range of tests, which may be beneficial for identifying a broader spectrum of bacteria, though it may require more time and resources
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