0
Invitrogen™ Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP, 1mL
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | Western Blot, ELISA-Based Assays, Immunohistochemistry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP is a high-quality polyclonal secondary antibody, specifically designed for the detection of mouse IgG (heavy and light chains) in Western blot (WB), immunohistochemistry (IHC), and ELISA applications. This antibody is conjugated to Horseradish Peroxidase (HRP), enabling chemiluminescent, colorimetric, and fluorescence-based detection in immunoassays.
The antibody is purified via antigen-affinity chromatography and formulated in a stabilizing buffer to enhance sensitivity and minimize non-specific binding, making it an ideal choice for researchers requiring high signal and low background in protein detection applications.
Key Features & Benefits
✔ High Sensitivity & Low Background – Affinity-purified for superior specificity and minimal cross-reactivity.
✔ HRP Conjugation for Chemiluminescent/Colorimetric Detection – Ideal for Western blotting, ELISA, and IHC.
✔ Broad Application Use – Detects Mouse IgG (H+L) with increased signal amplification.
✔ High Stability – Formulated in PBS with stabilizers (BSA and glycerol) for long-term storage and enhanced performance.
✔ Versatile Dilution Range – Recommended 1:2,000 - 1:10,000 dilution depending on application.
Applications & Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 - 1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000 - 1:4,000 |
| ELISA (ELISA) | 1:2,000 - 1:4,000 |
✔ Compatible with chemiluminescent (ECL), colorimetric (TMB, DAB), and fluorogenic substrates for visualization in WB, ELISA, and IHC.
Product Specifications
| Feature | Details |
|---|---|
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Reactivity | Mouse IgG (H+L) |
| Conjugate | Horseradish Peroxidase (HRP) |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Immunogen | Mouse gamma immunoglobulins (IgG heavy and light chains) |
| Storage Buffer | PBS, pH 7.4, with 4 mg/mL BSA, 40% glycerol, and 0.19% Kathon™ CG |
| Purification Method | Affinity purified |
| Storage Temperature | 4°C (Do NOT freeze) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| Regulatory Status | For Research Use Only (RUO) |
Target Information
The Goat anti-Mouse IgG (H+L) Secondary Antibody binds specifically to the heavy and light chains of mouse IgG, making it a versatile detection tool in a variety of immunoassays.
- Western Blot (WB): Detects mouse primary antibodies in protein analysis studies.
- Immunohistochemistry (IHC): Used in tissue staining applications for detecting mouse IgG primary antibodies.
- ELISA: Facilitates antibody-based quantification of proteins and antigens.
Storage & Stability
📦 Storage Temperature: 4°C (Refrigerated) upon receipt.
❄ Do NOT freeze, as this may affect antibody activity.
⚠ Shelf Life: Long-term stability when stored under recommended conditions.
Safety & Handling
⚠ For Research Use Only (RUO) – Not for diagnostic, therapeutic, or clinical applications.
⚠ Wear PPE – Gloves, lab coat, and eye protection recommended.
⚠ Dispose of waste properly according to laboratory safety protocols.
Related Products & Alternative Secondary Antibodies
| Catalog # | Product Name | Conjugate | Unit Size |
|---|---|---|---|
| A31571 | Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody | Alexa Fluor™ 647 | 1 mg |
| 616520 | Rabbit anti-Mouse IgG (H+L) Secondary Antibody | HRP | 1 mL |
| 31160 | Goat anti-Mouse IgG (H+L) Secondary Antibody | Unconjugated | 2 mg |
| A11030 | Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody | Alexa Fluor™ 546 | 1 mg |
| F2761 | Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody | FITC | 1 mg |
| A28175 | Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody | Alexa Fluor™ 488 | 1 mg |
| A21203 | Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody | Alexa Fluor™ 594 | 1 mg |
| A10521 | Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody | Cyanine3 | 1 mg |
The Invitrogen™ Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (Catalog # 62-6520) is a high-quality, high-affinity reagent designed for Western blotting, ELISA, and immunohistochemistry applications. Its strong HRP conjugation, high specificity, and optimized buffer formulation ensure low background noise and high signal intensity, making it a reliable choice for protein detection assays.