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Proteintech™ p53 Monoclonal Antibody (6C4B6), 150 µL
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Specifications:
| Application | Western Blot, immunocytochemistry, Immunohistochemistry, Immunoprecipitation (IP) | ||
| Storage Temperature | -20°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Proteintech | ||
| Product Grade | Molecular Biology | ||
The Proteintech p53 Monoclonal Antibody (6C4B6) is a high-quality mouse monoclonal antibody developed for the detection of p53 tumor suppressor protein in human, mouse, and rat samples. Recognizing an immunogen derived from amino acids 1–232 of p53, this antibody is well suited for researchers studying cell cycle regulation, DNA damage response, apoptosis, tumor biology, and cancer-related signaling pathways.
This antibody has been validated for Western blotting and immunohistochemistry on paraffin-embedded tissue, offering strong performance and broad utility in oncology, molecular biology, and translational research workflows. With an antibody concentration of 1 mg/mL, purified by Protein A, and supplied in PBS with 50% glycerol, it offers reliable stability and convenient storage for research laboratories.
Key Features
- Specific for p53 tumor suppressor protein
- Monoclonal antibody clone 6C4B6
- Reacts with human, mouse, and rat
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Validated for:
- Western Blot (WB): 1:2,000–1:10,000
- Immunohistochemistry (Paraffin) [IHC (P)]: 1:1,600–1:6,400
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Suitable for studies in:
- Cancer biology
- DNA damage and repair
- Cell cycle arrest
- Apoptosis signaling
- Tumor suppressor pathways
- Supplied as liquid, unconjugated antibody
- Preserved with 0.02% sodium azide
- Store at -20°C
Applications
The Proteintech p53 antibody is ideal for:
- Western blot analysis of p53 expression
- Immunohistochemistry on paraffin-embedded tissue sections
- Research on TP53 mutations
- Studies of cellular stress responses
- Investigation of tumorigenesis and malignancy
- Analysis of p53-mediated transcriptional regulation
- Academic and pharmaceutical cancer research
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Proteintech p53 Monoclonal Antibody (6C4B6) |
| Catalog Number | 60283-2-Ig |
| Type | Antibody |
| Class | Monoclonal |
| Clone | 6C4B6 |
| Host / Isotype | Mouse / IgG2b |
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Amount | 150 µg |
| Volume | 150 µL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Preservative | 0.02% sodium azide |
| Storage Condition | -20°C |
| Shipping Condition | Wet ice (domestic); Dry ice (international) |
Immunogen Information
| Parameter | Details |
|---|---|
| Immunogen | p53 Fusion Protein Ag0698 |
| Immunogen Region | 1–232 aa |
| Encoded By | BC003596 |
Target Information
p53 is one of the most important tumor suppressor proteins in mammalian cells. It functions as a sequence-specific transcription factor activated by cellular stress such as DNA damage and nucleotide deprivation. Once activated, p53 regulates the expression of genes involved in cell cycle arrest, DNA repair, senescence, and apoptosis, including p21 and other checkpoint-associated proteins.
Alterations or mutations in TP53 are among the most common events in human cancer. Mutant p53 proteins often lose their tumor suppressor activity, allowing abnormal cells to continue proliferating. Because of its central role in cancer development, p53 remains one of the most widely studied biomarkers in oncology and cell biology research.
Advantages of This Antibody
- Reliable monoclonal specificity for p53 detection
- High-performance dilution range for efficient assay optimization
- Broad species reactivity across common experimental models
- Suitable for both protein expression studies and tissue-based detection
- Excellent fit for cancer, molecular, and translational research applications
Ordering Information
| Catalog Number | Product | Volume |
|---|---|---|
| 60283-2-Ig | Proteintech p53 Monoclonal Antibody (6C4B6) | 150 µL |
The Proteintech p53 Monoclonal Antibody (6C4B6) is a dependable research reagent for laboratories investigating tumor suppression, cancer progression, apoptosis, and cell cycle control. With validated WB and IHC(P) performance, strong species reactivity, and high-quality formulation, it is an excellent choice for researchers requiring consistent and specific detection of p53.