Agilent High sensitivity D1000 reagents, for 112 DNA Samples

The Agilent High Sensitivity D1000 Reagents Kit is designed for precise and reproducible analysis of low-concentration DNA fragments ranging from 35 to 1000 base pairs (bp). These reagents are optimized for use with Agilent 4150 and 4200 TapeStation Systems, allowing automated, high-sensitivity DNA quality control (QC) for next-generation sequencing (NGS), PCR fragment analysis, and genomic integrity assessment.

This kit includes a DNA ladder and sample buffer sufficient for processing 112 samples, ensuring accurate and reliable fragment size determination and quantification in high-throughput workflows.

Key Features & Benefits

1. High-Sensitivity DNA Analysis from 35 to 1000 bp
   • Detects low-concentration DNA samples with minimal input volume.
   • Provides accurate fragment size distribution for library QC and PCR products.
2. Pre-Calibrated for Agilent TapeStation Systems
   • Ensures consistent, automated electrophoresis results.
   • Eliminates manual gel preparation and sample handling errors.
3. Includes DNA Ladder & Sample Buffer for 112 Samples
   • Provides a complete reagent set for streamlined DNA analysis.
   • Pre-mixed reagents minimize preparation time and handling variability.
4. Optimized for NGS, PCR QC & Low-Input DNA Samples
   • Ideal for small DNA fragment analysis in sequencing and amplification workflows.
   • Ensures reliable quality control for cell-free DNA, bisulfite sequencing, and forensic DNA applications.
5. Standardized DNA Integrity & Fragment Analysis
   • Produces high-resolution electropherogram traces for clear fragment visualization.
   • Provides precise quantification for reproducible data interpretation.

Product Specifications

Use Cases & Applications

1. Next-Generation Sequencing (NGS) Library Quality Control

• Ensures High-Quality DNA Input for NGS Workflows
  • Verifies library preparation efficiency by detecting adapter dimers and incomplete ligation products.
  • Confirms DNA fragmentation size distribution before sequencing.
• Optimized for DNA Library QC & Low-Input DNA Analysis
  • Suitable for NGS libraries from bisulfite sequencing, ATAC-seq, and ChIP-seq.
  • Enables precise DNA integrity assessment for sensitive sequencing applications.

2. PCR Product Validation & Amplicon Size Determination

• Confirms PCR Fragment Sizes for Cloning & qPCR Analysis
  • Differentiates specific amplicons from non-specific bands.
  • Ensures accurate product verification in multiplex PCR.
• High-Sensitivity Detection of DNA Amplification Products
  • Detects low-abundance DNA fragments in qPCR and endpoint PCR.
  • Ideal for forensic DNA applications and microbial DNA analysis.

3. Genomic DNA & Cell-Free DNA (cfDNA) Analysis

• Assess cfDNA Fragmentation for Liquid Biopsy Studies
  • Identifies nucleosomal cfDNA patterns in cancer diagnostics.
  • Detects low-concentration circulating DNA for disease biomarker research.
• DNA Integrity Assessment for Genomic Studies
  • Measures fragmentation levels in genomic DNA for sequencing applications.
  • Supports DNA QC for long-read sequencing and structural variant analysis.

4. Epigenetics & Low-Input DNA Research

• Ideal for Bisulfite Sequencing & Methylation Studies
  • Provides accurate DNA integrity analysis for epigenetic profiling.
  • Detects DNA degradation caused by bisulfite treatment.
• Facilitates Cancer Genomics & Tumor DNA Analysis
  • Ensures DNA quality control for detecting tumor-derived DNA fragments.
  • Used in high-sensitivity assays for rare variant detection.

Instructions for Use

Step-by-Step Protocol for TapeStation Analysis

1. Prepare Samples & Reagents:
   • Ensure reagents (DNA ladder and sample buffer) are at room temperature before use.
   • Load 1–2 µL of sample into the TapeStation tray.
2. Load the TapeStation System:
   • Insert the appropriate D1000 High Sensitivity DNA ScreenTape device.
   • Add prepared DNA samples to the designated wells.
3. Run the TapeStation Analysis:
   • Start the automated electrophoresis process via TapeStation software.
   • Each sample is analyzed in 1–2 minutes.
4. Interpret & Export Data:
   • Review electropherogram traces and gel-like images for DNA fragment analysis.
   • Export data as PDF, CSV, or LIMS-compatible formats.

Storage & Handling Instructions

• Storage Conditions:
  • Store at 4–25°C, protected from light.
  • Do not freeze, as it may affect reagent performance.
• Handling Precautions:
  • Use aseptic techniques when handling DNA samples.
  • Ensure reagents remain sealed until use to prevent contamination.
• Disposal Guidelines:
  • Dispose of used reagents as per laboratory biosafety regulations.
  • Follow institutional waste management protocols for electrophoresis consumables.

Why Choose Agilent High Sensitivity D1000 Reagents?

✅ Rapid, high-sensitivity DNA quality assessment for NGS & PCR workflows

✅ Preloaded reagents ensure consistency & minimize sample preparation time

✅ Compatible with Agilent 4150 & 4200 TapeStation Systems

✅ Accurate fragment size analysis from 35 to 1000 bp

✅ Reproducible, automated DNA QC with minimal hands-on time

The Agilent High Sensitivity D1000 Reagents Kit is a critical tool for DNA quality control in genomics, sequencing, and molecular biology applications, ensuring high-precision DNA fragment analysis with minimal sample handling.