Applied Biosystems™ PrepFiler™ Forensic DNA Extraction Kit, 100 Preps (updated)

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    Specifications:
    Application Nucleic Acid Extraction, Forensics Human identification
    Storage Temperature Room Temperature
    Product Type Nucleic Acid Kits Forms Kit with Various components
    Product Brand Thermo Fisher Scientific™, Applied Biosystems™
    Product Grade Molecular Biology

    The Applied Biosystems™ PrepFiler™ Forensic DNA Extraction Kit is a robust magnetic bead–based solution engineered for high-efficiency purification of DNA from forensic samples. Designed for reliability in both routine and challenging casework, this kit utilizes an optimized multi-component surface chemistry to maximize DNA yield, purity, and concentration.

    Ideal for forensic laboratories handling body fluids, stains, and swab samples, the PrepFiler™ system ensures effective removal of PCR inhibitors, enabling superior downstream performance in STR analysis and genotyping workflows. The kit is configured for manual extraction using 1.5 mL tubes, delivering consistent and reproducible results.

    Key Features & Benefits

    Superior DNA Yield & Purity

    • Advanced magnetic particle chemistry ensures high DNA recovery from low-template and degraded samples
    • Efficient removal of inhibitors improves PCR amplification success

    Optimized for Forensic Applications

    • Compatible with a wide range of forensic sample types:
      • Blood, semen, saliva
      • Stains and swabs of body fluids
    • Enhances reliability in critical casework samples

    Improved Downstream STR Performance

    • Produces highly purified DNA suitable for STR profiling and forensic genotyping
    • Supports improved data quality and interpretation accuracy

    Streamlined Workflow

    • Simplified manual protocol for efficient sample processing
    • Designed to handle diverse and complex sample matrices

    Enhanced Kit Configuration

    • Includes PrepFiler Wash Buffer B to reduce detergent carryover and PCR inhibition
    • All reagents supplied in one box with a single expiration date
    • Ambient shipping and storage, simplifying logistics

    Flexible Workflow Integration

    • Part of a complete forensic DNA workflow ecosystem from Applied Biosystems
    • Compatible with downstream amplification kits such as GlobalFiler™

    Applications

    • Forensic DNA extraction and purification
    • Criminal investigations and casework analysis
    • Human identification and databasing
    • STR profiling workflows in forensic laboratories

    Technical Specifications

    ParameterSpecification
    Product LinePrepFiler™
    Catalog Number4463351
    Number of Reactions100
    ApplicationForensic DNA Extraction
    Sample TypeForensic samples (body fluids, stains, swabs)
    FormatTube / Filter Column
    Isolation TechnologyMagnetic Bead
    ThroughputManual (not high-throughput)
    Shipping ConditionRoom Temperature
    Storage Temperature18–25°C
    Unit Size1 Kit

    Kit Contents

    • PrepFiler Lysis Buffer
    • PrepFiler Magnetic Particles
    • PrepFiler Wash Buffer A (Concentrate)
    • PrepFiler Wash Buffer B (Concentrate)
    • PrepFiler Elution Buffer

    Note:

    Plastic consumables (filter columns and spin tubes) are not included.

    For kits with plastics, refer to Cat. No. 4463350 or accessories (e.g., Cat. No. A36853).

    Unique Advantages

    • Proven forensic-grade chemistry trusted globally
    • High recovery from challenging and degraded samples
    • Reduced PCR inhibition with dual wash buffer system
    • Simplified logistics with ambient shipping and storage
    • Fully compatible with integrated forensic DNA workflows


    The PrepFiler™ Forensic DNA Extraction Kit (4463351) is a reliable and high-performance solution for forensic laboratories requiring consistent, high-quality DNA extraction. With optimized chemistry, improved inhibitor removal, and streamlined workflows, it ensures maximum DNA recovery and superior downstream STR analysis, making it an essential tool for modern forensic applications.

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