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Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Proteins & Peptides | Forms | Solid |
| Product Brand | R&D Systems | ||
| Product Grade | Analytical grade | Formula | C₄₄H₃₈N₈ · C₂₈H₂₈O₁₂S₄ |
High-Purity TMPyP4 for Telomerase Inhibition & G-Quadruplex DNA Studies
TMPyP4 Tosylate is a high-affinity telomerase inhibitor (IC₅₀ = 6.5 µM) that selectively binds to DNA G-quadruplex structures, stabilizing them and disrupting telomerase activity. It is widely used in cancer research, DNA structural studies, and small-molecule drug development.
This Calbiochem®-branded TMPyP4 (≥95%) is available in a solid form (purple color) and exhibits strong fluorescence in the presence of quadruplex DNA, making it a valuable tool in telomerase-targeted therapies, DNA-protein interaction studies, and fluorescence-based G4 assays.
Key Features & Benefits
✅ High-Purity Telomerase Inhibitor
- ≥95% purity, ensuring consistent biological activity in experiments.
- Potent inhibition of human telomerase (IC₅₀ = 6.5 µM), making it ideal for cancer and aging research.
✅ Specific Binding to DNA G-Quadruplexes
- Stacks on G-tetrads at the core of quadruplex DNA, stabilizing G4 structures.
- Fluoresces intensely in the presence of quadruplex DNA, enabling biophysical and fluorescence-based assays.
✅ Validated in Cancer Research & DNA Targeting
- Cited in multiple peer-reviewed studies (Cancer Res., Biochemistry, Nucleic Acids Res., J. Am. Chem. Soc.).
- Used to study telomerase inhibition, DNA structural biology, and potential anticancer therapies.
✅ Reliable & Reproducible
- Manufactured by Calbiochem®, a trusted supplier of biochemical reagents.
- Solid form for precise dosing and long-term stability.
✅ Safe Handling & Storage
- Stable at 2–8°C, protected from light and moisture.
- Non-cell permeable, allowing targeted extracellular and in vitro studies.
Technical Specifications
| Property | Details |
|---|---|
| Chemical Name | TMPyP4 Tosylate |
| Purity | ≥95% (TLC) |
| Molecular Formula | C₄₄H₃₈N₈ · C₂₈H₂₈O₁₂S₄ |
| Molecular Weight | 1363.60 g/mol |
| CAS Number | 36951-72-1 |
| Form | Solid (Purple Color) |
| Storage Conditions | 2–8°C (Desiccated, Light-Protected) |
| Solubility | Water: 1 mg/mL |
| Manufacturer/Brand | Calbiochem® (R&D Systems) |
| Fluorescence | Strong G-quadruplex DNA-dependent fluorescence |
| Stability | Long-term stable under recommended storage conditions |
| Shipping Conditions | Ambient |
| Toxicity | Standard Handling (A) |
Biochemical & Physiological Actions
- Selective inhibitor of human telomerase (IC₅₀ = 6.5 µM).
- Binds and stabilizes G-quadruplex (G4) DNA structures, preventing telomerase-mediated elongation.
- Does not compete with ATP, allowing targeted DNA-protein interaction studies.
- Irreversible inhibitor, leading to long-lasting telomerase suppression.
Use Cases & Applications
1. Telomerase Inhibition & Cancer Research
Primary Use:
- Investigating telomere-targeting anticancer therapies.
- Studying telomerase activity suppression in tumor cells.
Practical Example:
A cancer research lab uses TMPyP4 to inhibit telomerase activity in glioblastoma and breast cancer cell lines, exploring novel telomerase-targeting strategies for drug development.
2. G-Quadruplex DNA Structural Studies
Primary Use:
- Probing G4 formation, stability, and ligand interactions.
- Assessing quadruplex-binding small molecules for drug screening.
Practical Example:
A structural biology lab uses TMPyP4 fluorescence to visualize G-quadruplex DNA structures, confirming their role in genome stability and transcriptional regulation.
3. Fluorescence-Based DNA Binding & Biophysical Assays
Primary Use:
- Assessing G-quadruplex-binding affinity of novel ligands.
- Screening potential DNA-targeting small molecules for anticancer applications.
Practical Example:
A drug discovery team screens G4-binding compounds using TMPyP4 fluorescence intensity shifts as a readout, identifying lead candidates for telomerase inhibition.
4. Genomic & Epigenetic Regulation Studies
Primary Use:
- Evaluating the impact of G4 stabilization on gene expression.
- Investigating quadruplex-mediated transcriptional control in human cells.
Practical Example:
A genomics research lab explores how TMPyP4-stabilized G4 structures modulate c-MYC transcription, linking quadruplex formation to oncogene regulation.
Why Choose R&D Systems TMPyP4 Tosylate?
🧪 Highly Pure (≥95%) – Ensures reliable and reproducible experimental results.
🔬 Selective & Potent Telomerase Inhibitor – IC₅₀ = 6.5 µM, making it a valuable tool in cancer research.
⚡ G-Quadruplex Stabilization – Ideal for DNA structural studies and fluorescence assays.
📈 Cited in Peer-Reviewed Literature – Used in multiple high-impact research publications.
🛡 Stable & Easy to Handle – Long shelf life, with light and moisture protection for extended usability.
The R&D Systems TMPyP4 Tosylate (≥95%) is a high-purity, telomerase inhibitor and G-quadruplex stabilizer, widely used in cancer research, DNA structural studies, and fluorescence-based assays. With its proven efficacy in targeting telomerase activity (IC₅₀ = 6.5 µM) and strong fluorescence properties, TMPyP4 is an essential tool for telomere and DNA-targeting investigations.