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Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X), 10 mL
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Supplement | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ Insulin-Transferrin-Selenium (ITS-G) (100X) is a defined serum supplement designed to reduce or eliminate the requirement for fetal bovine serum (FBS) in mammalian cell culture.
ITS-G is routinely used at a 1X final concentration, typically in combination with 2–4% FBS, to support robust cell growth and maintenance across a broad range of cell lines, including epithelial, fibroblast, and lymphoid cells.
This supplement provides essential growth factors, trace elements, and antioxidants, helping maintain cell viability, metabolic activity, and consistent culture performance under serum-reduced conditions.
Key Features
- Serum Reduction: Supports healthy cell growth with minimal FBS supplementation.
- Broad Compatibility: Effective in RPMI 1640, MEM, and most common basal media.
- Nutrient-Rich Composition: Supplies insulin, transferrin, and selenium for metabolic and antioxidant support.
- cGMP Manufacturing: Produced in an FDA-registered, ISO 13485-certified facility in Grand Island, NY.
- Stable and Convenient: 100X concentrate; shelf life of 18 months from manufacture.
- Sterile and Phenol Red-free: Ensures minimal interference in sensitive assays.
- Research Use Only (RUO): Not intended for diagnostic or therapeutic applications.
Component Functions
- Insulin: Promotes glucose and amino acid uptake and supports macromolecule biosynthesis.
- Transferrin: Facilitates iron transport while minimizing oxidative stress caused by free radicals.
- Selenium (as sodium selenite): Acts as a cofactor for glutathione peroxidase, providing antioxidant protection and supporting redox balance.
Specifications
| Parameter | Specification |
|---|---|
| Cell Type | Mammalian cells |
| Concentration | 100X |
| Quantity | 10 mL |
| Formulation | Insulin, Transferrin, Selenium (no phenol red) |
| Sterility | Sterile-filtered |
| Shelf Life | 18 months |
| Storage Conditions | 2–8 °C |
| Shipping Conditions | Room temperature |
| Product Type | Basal medium supplement |
| Intended Use | Research Use Only (RUO) |
| Manufacturing Standards | cGMP, FDA-registered, ISO 13485 certified facility |
Applications
- Mammalian cell culture under serum-reduced conditions
- Long-term cell maintenance and metabolic regulation
- Serum-free and defined media development
- Epithelial, fibroblast, and hybridoma cell culture
- Oxidative stress reduction and cell protection in vitro
The Gibco™ ITS-G Supplement (100X) provides a balanced and defined serum alternative that ensures consistent and reproducible results in mammalian cell culture.
With insulin for nutrient uptake, transferrin for iron transport, and selenium for antioxidant protection, ITS-G reduces serum dependency while maintaining robust cell viability and productivity.
Manufactured under cGMP and ISO 13485 standards, this supplement delivers Gibco’s trusted quality for reproducible cell culture performance.