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Invitrogen™ Goat anti-Human IgG (H+L) Secondary Antibody, HRP
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Specifications:
| Application | Western Blot, immunocytochemistry, Immunohistochemistry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Goat anti-Human IgG (H+L) Secondary Antibody, HRP-conjugated (Catalog #31410) is a high-affinity polyclonal secondary antibody designed to detect human IgG molecules, including both the heavy (H) and light (L) chains. This antibody is conjugated to horseradish peroxidase (HRP), making it ideal for enzyme-based detection in a wide range of immunoassays, including Western blot (WB), immunohistochemistry (IHC), and immunocytochemistry/immunofluorescence (ICC/IF).
This antibody is particularly suited for research applications that require high sensitivity, specificity, and signal amplification, and is commonly used for target protein detection, diagnostic assay development, and biomarker research.
Key Features & Benefits
✅ Broad IgG Recognition
- Detects both heavy and light chains of human IgG, ensuring complete coverage of target antibodies.
- Reacts with IgG subclasses 1–4 and light chains (kappa and lambda).
✅ High Sensitivity with HRP Conjugation
- HRP enables chemiluminescent, colorimetric, or fluorometric detection, compatible with a range of substrates like ECL, DAB, and TMB.
- Suitable for low-abundance target detection with strong signal amplification.
✅ Affinity-Purified for Specificity
- Purified using antigen-specific affinity chromatography, reducing background and non-specific binding.
✅ Versatile Use Across Assays
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Validated for:
- Western blot (WB)
- Immunohistochemistry (IHC)
- Immunocytochemistry/Immunofluorescence (ICC/IF)
✅ Convenient Format
- Lyophilized for long-term storage.
- Supplied in 2 mL size; reconstitutes to 0.8 mg/mL.
Product Specifications
| Property | Details |
|---|---|
| Catalog Number | 31410 |
| Host / Isotype | Goat / IgG |
| Target | Human IgG (H+L) |
| Conjugate | Horseradish Peroxidase (HRP) |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL (after reconstitution) |
| Purification | Antigen-affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 15 mg/mL BSA |
| Preservatives | None |
| Storage Conditions | 4°C (short-term); –20°C (with glycerol) |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
| Reconstitution | Add 2 mL distilled water |
| RRID | AB_228269 |
| Country of Origin | USA |
Applications & Suggested Dilutions
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:10,000–1:200,000 |
| Immunohistochemistry (IHC) | 1:500–1:5,000 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:500–1:5,000 |
Target Specificity
- Binds to the Fc and Fab regions of human IgG heavy chains and light chains common to all immunoglobulins (IgG, IgA, IgM).
- Does not bind to non-immunoglobulin serum proteins.
- May cross-react with immunoglobulins of other species—use cross-adsorbed versions when specificity is critical.
Compatible Detection Systems
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HRP Substrates:
- Chemiluminescent: ECL, SuperSignal™
- Colorimetric: DAB, TMB
- Fluorescent: HRP-based fluorogenic substrates
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Compatible Membranes:
- PVDF
- Nitrocellulose
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Visualization:
- X-ray film, CCD imaging, or colorimetric visualization
Restoration & Storage Instructions
- Reconstitute lyophilized antibody with 2 mL distilled water.
- Allow to stand for 1–2 hours at room temperature. Centrifuge if cloudy.
- Short-term storage: 4°C as undiluted liquid.
- Long-term storage: Mix 1:1 with glycerol (final 50%) and store at –20°C.
- Avoid repeated freeze-thaw cycles.
Why Choose This Antibody?
✅ High sensitivity for IgG detection in WB, IHC, and IF
✅ HRP conjugate for versatile detection methods
✅ Validated across multiple applications and sample types
✅ Goat-derived, affinity-purified for specificity and low background
✅ Reliable performance from Invitrogen trusted by researchers globally
This antibody is perfect for researchers conducting protein expression analysis, diagnostic assay development, autoimmune disease research, or immunoglobulin profiling.