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Gibco™ Medium 199, Earle's Salts, powder, L-glutamine, HEPES, 50L
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With: L-glutamine, HEPES
Without: Phenol Red, Sodium bicarbonate
Specifications:
| Application | Cell culture | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Gibco | ||
| Product Grade | Cell Culture | ||
Gibco™ Medium 199 (M199) was originally formulated for the nutritional studies of chick embryo fibroblasts and has since proven to be widely applicable for cultivation of non-transformed cells across species. It is extensively used in virology, vaccine production, and the in vitro cultivation of primary explants such as mouse pancreatic epithelium and rat lens tissues.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins, which support robust cell growth and maintenance.
This formulation includes Earle’s salts, making it suitable for use in a CO₂ incubator environment, where bicarbonate buffering maintains physiological pH.
Key Features
- Broad Applicability – Supports a variety of primary cells and explants.
- Unique Nutrient Profile – Contains nucleic acid precursors (adenine, adenosine, hypoxanthine, thymine) and additional vitamins.
- Virology & Vaccine Research – Commonly used in virus culture and vaccine production workflows.
- Explants & Primary Cultures – Suitable for chick embryo fibroblasts, mouse pancreatic epithelium, and rat lens tissue.
- Powder Formulation – Requires supplementation with sodium bicarbonate, pH adjustment, and filtration prior to use.
- Manufacturing Quality – cGMP-compliant under ISO 13485 standards.
Composition Details
With:
- L-glutamine
- Phenol Red
Without:
- HEPES
- Sodium pyruvate
Buffer system: Sodium bicarbonate (2.2 g/L), requires 5–10% CO₂ for pH control.
Applications
- Nutritional and metabolic studies
- Virology and vaccine production
- Cultivation of primary explants (mouse pancreatic epithelium, rat lens)
- General cell culture of non-transformed cells
Technical Specifications
| Property | Details |
|---|---|
| Product Type | Basal cell culture medium |
| Form | Powder |
| Salts | Earle’s salts |
| With Additives | L-glutamine, Phenol Red |
| Without Additives | HEPES, Sodium Pyruvate |
| Buffer System | Sodium bicarbonate (2.2 g/L) |
| Required Atmosphere | 5–10% CO₂ |
| Cell Types Supported | Chick embryo fibroblasts, mouse pancreatic epithelium, rat lens |
| Packaging Size | 50 L |
| Catalog Number | 31100019 |
| Storage Conditions | 2–8 °C |
| Shipping Conditions | Ambient |
| Shelf Life | 36 months (from manufacture) |
| Compliance | cGMP, ISO 13485 |
Ordering Information
| Catalog Number | Quantity | Formulation | Unit Size |
|---|---|---|---|
| 31100019 | 50 L | Earle’s salts, powder | Each |
The Gibco™ Medium 199, Earle’s Salts, Powder is a nutrient-rich basal medium designed for primary cultures, virology research, and vaccine production. With its unique nucleic acid precursors and vitamin supplementation, it provides superior nutritional support for sensitive cells and explants. The powdered format ensures flexibility, making it an economical and versatile choice for research laboratories.